Hop variety named `Columbus`

ABSTRACT

A new and distinct variety of hop, Humulus lupulus L., named Columbus, has a superior yield of cones and a superior content of alpha acids in its resin. The new variety was cultivated as a result of a cross at a nursery near Prosser, Wash., United States, and has been asexually reproduced in and about Yakima, Wash., United States.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a continuation of U.S. patent application Ser. No. 08/159,859, filed Nov. 30, 1993, now abandoned.

TECHNICAL FIELD

The field of the present invention is hops.

BACKGROUND OF THE INVENTION

Of conventional hop varieties, there are at least twenty named commercially grown hop varieties in the United States today, and some seventy-five to eighty varieties that are grown around the world. The varieties differ as to assorted physical features such as vine color and structure, as well as cone number, location, size and shape.

Commercial varieties of hop are female hops that exist as a perennial rootstock that produces an annual flush of above-ground, or aerial, vegetation. The perennial rootstock is comprised of a woody crown with a number of main roots and sub-roots that are used for water uptake, nutrient uptake, and winter starch storage. Over the winter, the woody crown supports many buds that develop into main vines (shoots) in the spring, emerge from the ground, and extend to produce the annual above-ground vegetation. In nature, with the onset of frosts in the fall, the above-ground portion of the plant "dies-back" to ground level, and only the perennial rootstock survives into the following year.

A main vine consists of a single flexible cylinder that produces pairs of leaves at nodes separated by variable distances along the length of the vine. The length of main vine between a pair of nodes is known as an internode. A bud is produced in the junction between leaf and vine, which junction is also known by the botanical term axil. Such a botanical term, as well as other botanical terms herein, are generally known to those of skill in the art, and can also be found in standard botanical texts such as Simon, E.W., et al., Lawson's Textbook of Botany--Revised, University Tutorial Press Ltd., London, England, 14th Ed. 1966. The bud grows and develops into a lateral, also known as a side-shoot or side-arm, with the same basic vegetative structure as the main vine. The buds on the laterals develop into groups of flowers that later mature into cones (strobiles). Because the laterals produce and support the cones of the hop, the structure of the laterals strongly influences the number of cones produced and hence the ultimate commercial yield of a variety.

The cones are the useful brewing commodity, and are referred to as "hops" in the commercial industry. Each cone is a group of flowers (inflorescence) consisting of sepals and petals (bracts and bracteoles) mounted on a central strig (or rachis). The resultant structure resembles a small pine cone or fir cone but is non-woody and green in color. In nature, the bracteoles support the seed of the plant.

Complete descriptions of the botanical structure of the hop plant are given in Burgess, "Hops--Botany, Cultivation and Utilization," Weed Crop Series, Interscience Publishers, Inc., New York, 1964 and Neve, "Hops," von Nostrand Reinhold, New York, 1991.

A trellis of poles and wire, commonly 18 feet in height and known as a "high trellis," supports strings that the main vines climb. The main vines grow to the top of the trellis, where they produce the laterals that support the groups of flowers that develop into the cones. Typically, the laterals bear cones only in the upper parts of the plant (i.e., closer to the main vine); the distance from the main vine varies from one variety to another. Under an alternative growing system, the "HopUnion Low Trellis Hop Growing System," the vines are grown on a trellis of single rows of 10 feet in height, like wine grapes (the 10 foot trellis is referred to herein as a "low trellis"). (Lewis, "The HopUnion U.S.A., Inc.--Low Trellis Hop Production System," Proc. Tech. Comm. of the IHGC of the XXXVIIIth International Hop Congress, Hereford, U.K. Published Rijksstation voor Landbouwtechniek, Merelbeke/Gent, Belgium, 17-37, 1990.) Under either system, when the cones are physiologically mature (ripe), the cones are harvested, then dried and compressed into bales for market, where the cones are typically used for the productuion of beverages such as beers, ales and related alcohol-containing liquids.

Different varieties of hops have differences in the chemical constituents of the lupulin of their cones. Accordingly, chemical analysis of such differences can be useful in distinguishing one hop variety from another. Such analysis is generally performed by high pressure liquid chromatography ("HPLC") of the soft resins along with gas liquid chromatography ("GLC") of the essential oils. Recently, Kenny developed a key for differentiation of hop varieties based on chemical analysis of the soft resins. (Kenny, "Identification of U.S. Grown Hop Cultivars by Hop Acid and Essential Oil Analyses," J. Amer. Soc. Brewing Chemists 48(1):3-8, 1990.) Further, Peacock and McCarty, as well as Kenny, have developed keys for the differentiation of hop varieties based on chemical analysis of the essential oils. (Peacock and McCarty, "Varietal Identification of Hops and Hop Pellets," Master Brewer Assoc. of the Americas Technical Quarterly 27:81-85, 1992; Kenny, supra.) These keys are based on gross differences in the amounts of, and ratios between, major constituents, which differences are large enough to overcome variation inherent in the analytical analysis of biological material.

SUMMARY OF THE INVENTION

The present invention provides a new variety of hop (Humulus lupulus L.), which has been named `Columbus`. The new variety comprises a superior yield of cones and, as discussed below, a superior content of alpha acids in its soft resins. Columbus is particularly suited for the production of beverages such as beers, ales and related alcohol-containing liquids.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts a section of Columbus vine near harvest.

FIG. 2 depicts a low trellis planting of Columbus.

FIG. 3 depicts early season growth of Columbus with an internode length of about 3 inches.

FIG. 4 depicts Columbus on a low trellis and shows the density of the cones in the canopy (cones per lateral node).

FIG. 5 depicts the physical appearance of Columbus cones.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides a new variety of hop (Humulus lupulus L.), named `Columbus,` that has a superior yield of cones and a superior content of alpha acids in its soft resins. Columbus may be classed as a bitter hop (i.e., high in alpha acids content). Columbus was bred as the result of an open-pollination cross that was carried out in 1982 at a small hop nursery near Prosser, Wash. U.S.A, and was known by the identification number 9/17. Pursuant to growth, analysis, and testing, Columbus was identified and selected from numerous candidates arising from the cross. The exact parentage of Columbus is unknown. The breeding nursery that produced Columbus contained 20-30 female plants from which seed was collected en masse, and an approximately identical number of male plants were growing and flowering at the time that pollination occurred. Columbus is a diploid hop.

Set forth below are various characteristics that distinguish Columbus from conventional hop varieties. Columbus is initially distinguishable on the basis of its physical characteristics, including the location of its cones, the internode distance, number of cones per node, the yield of its cones, and the physical characteristics of the cones. Columbus is also distinguishable on the basis of its chemical characteristics, which are determined by chemical analyses of the soft resins and essential oils. Additionally, methods for asexual reproduction of Columbus are discussed.

I. Physical Characteristics of Columbus A. Location of Cones

Columbus bears a high number of cones on its laterals down to a height of about 4 feet from the ground, when grown on a high trellis. A close-up Figure depicting in detail the vine structure of Columbus is presented as FIG. 1. Conversely, most conventional hop varieties on a high trellis do not bear significant numbers of cones below about 8 feet from the ground.

On a low trellis, Columbus produces significant numbers of cones down to ground level, and cones can even be found on untrained side-shoots running along the ground. (FIG. 2.) Conversely, most conventional hop varieties on a low trellis produce cones down to about 4 to 8 feet from the ground.

B. Internode Distance

The internode distance on a main vine of any hop variety is important to the yield of cones because the shorter the internode length, the greater the number of yielding laterals for a given length of main vine. Columbus has a shorter than average mature internode length, about 8" to 12", with an early season internode distance of about 3". (FIG. 3.) Conversely, in conventional hop varieties such as Nugget, the mature internode length varies from 10" to 16", with an early season internode distance of about 8". There are also "dwarf hops" in which the mature internode length varies between 5" to 8".

C. Growth Characteristics

Although the early season growth rate and length of time for hop vines to reach the top wire of a trellis vary widely due to factors such as climatic conditions and growth and harvest management, the early season growth of Columbus is generally faster than the most aroma hops (such as Tettnang, Perle, Centennial, Saaz, Spalt, Mt. Hood, Liberty, Hallertau, Hersbruck, and Fuggle), and also faster than the quicker-growing aroma hops such as Cascade and Willamette. Such aroma hops are generally first trained between May 7 and May 10, and reach the top wire between June 20 and June 30. The aroma hop Cluster and the bitter hops Galena and Chinook are typically trained about May 15 and reach the top wire from about June 20 to about June 30. The bitter hops Nugget, Olympic, Eroica, and Columbus are typically trained about May 25 and reach the top wire about June 27 to about July 7. Among this latter group, Columbus typically grows slower than the other hop varieties at first, but then accelerates its growth and can grow to the top faster than Nugget on occasion. In other words, Columbus is a late maturing hop, and has an early growth rate that is typical for bitter hops and for Cluster, but it is a slow starter.

D. Habit

In common with most other hop varieties, the main vines of Columbus are originally green in color but turn to dark red to purple as the season progresses. The main vines are ridged, and along the ridges are hooked hairs that adhere to the supporting trellis. A characteristic feature of Columbus vines, as discussed above, is the short internodes.

Although the lateral length of a hop variety can vary greatly due to factors such as climate and management practices, the laterals of Columbus are typically longer than those of aroma varieties, are typically similar in length to those of Galena and Nugget, and are typically shorter than Cluster, Chinook, Olympic and Eroica. A generalization of comparative lateral lengths is as follows:

                  TABLE 1     ______________________________________     Hop Variety          Length of Lateral     ______________________________________     Mt. Hood, Liberty, Saaz, Spalt, Perle,                          1'-2'     Hallertau, Tettnang, Hersbruck, Fuggle,     Centennial     Cascade, Willamette  1.5'-2.5'     Columbus, Galena, Nugget                          2.0'-3.0'     Cluster, Chinook, Olympic, Eroica                          2.5'-3.5'     ______________________________________

Although most commercial hops have longer laterals in the head and much shorter laterals in the lower reaches of the vine. Columbus tends to have approximately even length laterals along the length of the vine. The sub-branches of the laterals develop as bunches of cones, and as set forth below, Columbus has an unusually high number of cones in each lateral bunch, typically greater than any other commercial variety.

The head of the vine is also subject to variation depending on such factors as climatic conditions and management practices, but Columbus can be generally grouped with Cascade. Willamette, Nugget and Galena as having a moderate head, although the head of Columbus is slightly smaller and neater than typical. The head of Columbus also appears more dense than most other varieties, apparently because more laterals are developed from an equivalent vine length due to the short internode distance discussed above.

In terms of overall vine morphology, Columbus tends to be stronger than the aroma varieties but slighter than the other bitter hops and Cluster. As noted above, the overall vine morphology also gives the appearance of being more dense than other commercial hop varieties.

E. Leaf Morphology

The leaf morphology of Columbus is typical for most commercial hop varieties, and may be summarized as set forth in the table below.

                  TABLE 2     ______________________________________              Early Main Vine                          Late Main Vine                                       Lateral     Character              Leaves      Leaves       Leaves     ______________________________________     Number   2/node      2/node       2/node     Lobes    cordate - 3 35           cordate - 3     Width    3-5"        4-7"         1-2"     Length   3-5"        4-7"         1-2"     Upper Surface              smooth      smooth       smooth     Texture     Lower Surface              fairly rough                          rough        slightly rough     Texture     Color    med-dark green                          dark green   med-dark green     Margins  lightly serrated                          moderately serrated                                       lightly serrated     ______________________________________

The leaf color of Columbus is also similar to other commercial hop varieties, although in the early part of the growing season when Columbus is slower growing than other bitter hops and Cluster, Columbus has a darker leaf, while later in the season when Columbus is faster growing, the leaf of Columbus is similar to other hops in its class. The leaf of Columbus is typically not as dark as most of the slower growing aroma hops.

F. Number of Cones per Node

Typically, cones are found in high number clusters borne at nodes on a lateral that are close to the main vine ("basal lateral nodes"). Progressively fewer cones are found in the clusters located at nodes progressively farther away from the main vine. Ultimately, the lateral terminates in a tip bearing a single cone. Columbus yields a high number of cones at the basal lateral nodes as well as at nodes located farther away from the main vine. (FIG. 4.) The basal lateral nodes of Columbus have produced up to 42 cones. Conversely, conventional low yielding hop varieties typically bear clusters of about 8-16 cones at basal lateral nodes, while conventional high yielding varieties typically bear clusters of about 16-28 cones at basal lateral nodes.

G. Yield of Cones

Due to the above discussed characteristics, Columbus provides an outstanding hop variety comprising the following: a plant bearing cones much lower down the main vine; a reduced internode length on the main vine, which results in a greater number of yielding laterals per given length of main vine; and, greater numbers of cones at each node, which results in greater numbers of cones on each of the yielding laterals. Accordingly, Columbus produces an outstanding yield of cones, whether grown on a high trellis or a low trellis.

Yield data for many hop varieties are compared with yield data for Columbus in Table 3. All hops were grown on a high trellis.

                  TABLE 3     ______________________________________                  Washington State                               HopUnion                    Five-Year Average                               1992 Average     Variety       Yield (lb/acre)                                 Yield (lb/acre)     ______________________________________     Low yielding varieties     Perle                1172              1366     Tettnang           970               1140     High yielding varieties     Cluster              2018              2407     Chinook              1938              2216     Nugget                1950                                            2829     Galena                1926                                            2502     Columbus             --               3290     ______________________________________

(Hop Growers of America, Inc., 1991-1992 Annual Statistical Report, 37th Annual HGA Convention, Portland, Oreg., 1993.)

Below in Table 4, yield data from low trellis hops grown at HopUnion farms for 1990 and 1992 are compared with experimental yield data for Columbus from 1991 and 1992.

                  TABLE 4     ______________________________________     Yield Data for Low Trellis Hops                  1990 Average                             1992 Average     Variety       Yield (lb/acre)                               Yield (lb/acre)     ______________________________________     Perle        1557       1010     Tettnang          1062              800     Cascade              1415                                          1570     Chinook              1234                                          1017     Nugget                1712                                          1175     Galena                1800                                          1500     (Year: 1991)     Columbus     2832       3408     ______________________________________

First year plantings of hops typically yield significantly less than mature plantings of the same varieties. However, a 1992 first year planting of Columbus on a low trellis yielded, over an area of about 4.5 acres, an average of 2547 lb/acre. Columbus produces such yields with a growth habit that presents no problems for ease of harvesting.

H. Physical Characteristics of the Cones

Cones are typically described by size (large, medium, or small) and by degree of compactness (loose, medium, or tight). Size and compactness are indicative of the degree of compression (internode length) between the nodes of the central strig that subtends (supports) the bracts and bracteoles. Also, cones are often described in terms of weight, most often expressed in milligrams (mg). Light cones are usually between 120 and 160 mg in weight, heavy cones usually between 160-220 mg (kiln dry basis). Often the description includes a brief comment on general shape of the cone, such as round, ovoid, plump, etc.

Columbus cones are normally medium in length, typically about 1.25", medium to tight in compactness, and typically about 1" in diameter. Further, Columbus cones are typically quite rounded and plump, and weigh about 180-250 mg (kiln dry basis) at maturity. (FIG. 5.) Conversely, Chinook cones typically have bracts with the tips turned backwards (reflexed). Fuggle cones are typically square in cross-section. Nugget cones are typically compact, tight, and firm. Columbus cones are typically more dense than other commercial hop varieties, and hence typically heavier on a per cone basis. Columbus cones have little or no propensity to shatter when fresh or when properly dried.

I. Disease and Pest Resistance

The susceptibility of Columbus to downy mildew (Pseudoperunospora humuli) is similar to Galena and Cluster, which are less resistant than most other commercial hop varieties. Columbus is apparently resistant to Verticillium wilt (Verticillium dahliae). Tests have indicated that Columbus can become infected with Prunus necrotic ringspot virus, hop latent virus and American hop latent virus, although it is also possible to free Columbus of these viruses by standard meristem culture techniques. Columbus has not shown any symptoms of infection when carrying these viruses, as is usual for most commercial hop varieties. Columbus shows no unusual tolerance or sensitivity to damson hop aphid (Phorodon humuli) and two spotted mite (Tetranychus urticae).

J. Quality Profile

Based upon sensory analyses of dried/baled leaf hop samples, Columbus has a quality profile that is similar to most other commercial hop varieties. Columbus has a mild, gentle aroma with no particular, peculiar character. However, Columbus is distinguishable from the commercial aroma varieties and Cluster by virtue of its higher resin content, which is indicated by visual inspection of the open cone and the much greater number of lupulin glands present in Columbus.

II. Chemical Characteristics of Columbus A. Chemical Analyses in General

Chemical analysis of hops typically consists of analysis of the lupulin of the cones. The lupulin consists of the following chemical components and sub-components:

    ______________________________________     Soft resins       alpha acids (humulones)                                           beta acids (lupulones)     Hard resins     Uncharacterized resins     Waxes     Tannins     Essential oils        hydrocarbons                                         oxygenated compounds                                         sulfur compounds     ______________________________________

Of the above components, the soft resins and essential oils are of particular relevance to the differentiation of hop varieties. In regard to the soft resins, there are three analogues of the alpha acids, i.e., humulone, ad-humulone, and cohumulone, and there are also three analogues of the beta acids, i.e., lupulone, ad-lupulone, and colupulone.

Due to their chemical characteristics, some hop varieties are referred to as aromatic, or low alpha, hops and are known to contribute good hop flavor and aroma. Other hop varieties are high in bittering potential (i.e., high in alpha acids) and are referred to as bitter or high alpha hops. Columbus may be referred to as such a bitter or high alpha hop. Hop varieties in an intermediate varietal classification have medium levels of alpha acids and are referred to as dual-purpose hops.

B. Soft Resin Characteristics

Table 5 lists the total amount of alpha and beta acids, the ratio of alpha acids to beta acids, and estimates of the relative proportions of cohumulone and colupulone for several high alpha hops. This data was determined by HPLC analysis according to standard methods well known to those having skill in the art. (See, e.g., American Society of Brewing Chemists, "Hops Method 12: Analysis of Hops, Hop Powders and Hop Pellets by High Pressure Liquid Chromatography," in "Methods of Analysis," The Society, St. Paul, Mn., 8th ed. 1991.) Table 5 also gives data on storage losses, which indicate the loss of alpha acids by oxidation. The data are expressed as ranges to account for inherent biological variation from sample to sample.

                  TABLE 5     ______________________________________     Parameter     Chinook   Nugget   Galena     ______________________________________     Alpha acids (% w/w)                   12.0-14.0 12.0-14.0                                      11.0-13.0     cohumulone (%)                            30-35                                 24-30                                             36-42     Beta acids (% w/w)                        3.4-4.0                                  4.0-5.5                                       7.0-9.0     colupulone (%)                             55-66                                     50-55                                            60-65     Alpha/beta ratio                            3.5-4.0                                  2.5-3.0                                         1.5-2.0     Oil content (mls/100 g)                      1.5-2.3                                  1.2-2.0                                         0.7-1.2     Loss of alpha acids (%)                        35-45                                    20-35                                              20-30     6 months storage     room temperature     ______________________________________     Parameter     Olympic   Eroica   Columbus     ______________________________________     Alpha acids (% w/w)                   11.5-13.5 11.0-13.0                                      14.0-16.0     cohumulone (%)                                 38-43                                             30-35     Beta acids (% w/w)                        4.0-6.0                                  4.0-5.5                                       4.5-5.5     colupulone (%)                             57-62                                     67-70                                            55-60     Alpha/beta ratio                            2.0-2.7                                  2.2-2.8                                         2.7-3.3     Oil content (mls/100 g)                      1.4-2.0                                  0.5-1.3                                         1.5-2.2     Loss of alpha acids (%)                        40-55                                    35-50                                              60-80     6 months storage     room temperature     ______________________________________

Table 5 indicates the following: (a) Columbus has a higher content of alpha acids than the other high alpha hops; (b) Columbus' cohumulone and colupulone contents are higher than Nugget, lower than Eroica and Galena, and similar to Chinook; (c) Columbus' beta acid content is higher than Chinook and lower than Galena; (d) Columbus' alpha/beta ratio is higher than Galena or Olympic but lower than Chinook; and (e) Columbus more rapidly loses its alpha acids in ambient storage than the other varieties.

Table 5 shows that Columbus has an alpha acids content well in excess of 10% w/w. This distinguishes Columbus from the aromatic hops. Examples of such aromatic hops are listed in Table 6.

                  TABLE 6     ______________________________________                  Alpha acids content     Variety        (% w/w of cone)     ______________________________________     Cascade      4.5-7.0     Cluster                    5.5-8.5     Fuggle                       4.0-5.5     Hallertau                 3.5-5.5     Hersbruck                 3.5-5.5     Liberty                      3.0-5.0     Mount Hood               5.0-7.5     Perle                         7.0-9.5     Saaz                            3.0-4.5     Spalt                           3.0-5.5     Tettnang                     4.0-5.0     Willamette                  4.0-6.0     ______________________________________

As discussed above, Kenny developed a key for identification of hop varieties based on the soft resin components. The application of the key of Kenny to Columbus is depicted in Table 7. At each decision point, certain varieties are eliminated according to a yes/no decision. The path to Columbus is shown by the double arrow. In the key depicted in Table 5, as well as in the other keys discussed herein, steps from the published key that did not eliminate any varieties in the present analysis have been deleted.

                  TABLE 7     ______________________________________                     Major U.S. Variety Eliminations     ______________________________________     1.  Fresh alpha + beta > 15%                         →                               NO   Cascade, Cluster,         and Fresh Alpha > 10%      Fuggle, Hallertau,                                    Hersbruck, Liberty,     .arrow-down dbl.            YES               Mt. Hood, Perle, Saaz,                (High alpha hops)                              Spalt, Tettnang,                                            Willamette     2.  Cohumulone > 40%                         →                               YES  Eroica     .arrow-down dbl.            NO     3.  Colupulone > 60%                         →                               YES  Galena     .arrow-down dbl.            NO     4.  Cohumulone < 30%                         →                               YES  Nugget     .arrow-down dbl.            NO     5.  Alpha/beta ratio > 3.0                         →                               YES  Chinook (3.5-4.0)     .arrow-down dbl.    →                               NO   Olympic (2.0-2.7)     Columbus(2.7-3.3)     ______________________________________

Table 7 relates to hops with a hop storage index, or HSI, well below 0.300, which indicates that the alpha and beta acids of the hops are fresh when analyzed. In Table 7, step 1 eliminates all the aromatic hops on the basis of their low content of alpha acids and low alpha plus beta acids. Step 2 eliminates Eroica on the basis of high cohumulone content. Step 3 eliminates Galena on the basis of high colupulone content. (Eroica is again eliminated at this step.) Step 4 eliminates Nugget on the basis of low cohumulone content. Step 5 eliminates Chinook on the basis of a high alpha/beta ratio, while Olympic is eliminated on the basis of a low alpha/beta ratio. Note, Chinook is also eliminated on the basis of its higher beta acid content.

Accordingly, Columbus is differentiated from all major U.S. varieties based on its soft resins content. Similarly, Columbus is distinguished from the minor U.S. hop varieties, except Aquila, Banner, and Centennial, based on soft resins content. However, Columbus is distinguished from Aquila, Banner, and Centennial by its essential oils content, as discussed below.

C. Essential Oils Characteristics

Detailed analysis of the essential oils allows a further differentiation of Columbus from other hops. Table 8 further distinguishes some of the varieties discussed above in Table 7, while Table 9 distinguishes varieties not distinguished above.

Extraction and analysis of essential oils are performed according to methods well known to those having skill in the art. Preferably, the essential oils are extracted by steam distilling previously dried hops for about four hours (with the steam of the distillation condensed using a cold-finger condenser). The resultant essential oils are then examined by GLC analysis.

GLC analysis for various hops are presented as Appendices 1 and 2 hereto as follows:

Appendix 1: Detailed essential oil analysis results of many of the world's hop varieties including all U.S. grown varieties except Mount Hood. Columbus is the final entry.

Appendix 2: Detailed GLC traces and listings of constituent peaks from essential oils analysis. Columbus is compared with the other U.S. high alpha hops, Galena, Chinook, and Nugget.

As with the soft resins, keys can be used to differentiate by essential oils content. Only United States varietal differentiations are depicted below in Tables 8 and 9, but non-United States varietal eliminations are listed in Peacock and McCarty, supra, and can be readily utilized by those having skill in the art to distinguish between those varieties and Columbus.

                  TABLE 8     ______________________________________     Key of Kenny                    Major U.S. variety eliminations     ______________________________________     1.  β-Farnesene content > 1%                          →                                YES  Cascade, Fuggle,     .arrow-down dbl.              NO               Saaz, Spalt                               Tettnang, Willamette     2.  Humulene/        →                                NO   Eroica, Olympic         β-Caryophyllene ratio > 1.5     .arrow-down dbl.              YES     3.  Presence of 2-Tridecanone/                          →                                NO   Galena         presence of a peak @        (*see note in text)         34.38 > 1.0%     .arrow-down dbl.              NO     4.  β-Caryophyllene/                          →                                YES  Cluster, Nugget         ∝-copaene > 20     .arrow-down dbl.              NO     Chinook  Columbus     (12.1)     (13.4)     ______________________________________

Table 8 was prepared using the data provided in Appendix 1. As shown in Table 8, step 1 eliminates the high β-farnesene hops, i.e., Cascade, Fuggle, Saaz, Spalt, Tettnang, and Willamette. Step 2 eliminates the low humulene varieties, i.e., Eroica and Olympic. Step 3 eliminates Galena because Columbus is unique for having no 2-tridecanone, and Columbus lacks a large peak found in Galena at 34.38 minutes (the peak is greater than 1% of the measured components of Galena). Step 4 eliminates both Cluster and Nugget. The remaining varieties are Columbus and Chinook. As shown in Table 7, Columbus is differentiated from Chinook by Columbus' lower alpha/beta ratio. In regard to some other varieties, Perle is eliminated by virtue of its low alpha acids content, while Columbus is distinguishable from Hersbruck, Hallertau, Liberty, and Mount Hood because Columbus has a much higher alpha acids content.

                  TABLE 9     ______________________________________     Key of Peacock and McCarty (1992)                  Major U.S. variety eliminations     ______________________________________     Columbus     .arrow-down dbl.     1.  Humulene present                        →                              YES  Aquila, Eroica     .arrow-down dbl.              YES     2.  β-Farnesene >                        →                              YES  Cascade, Fuggle         5% Humulene               Saaz, Spalt,                                   Tettnang, Willamette     .arrow-down dbl.              NO     3.  Humulene/Selinene > 20                        →                              YES  Banner, Hallertau,                                   Liberty, Mt. Hood,                                   Perle     .arrow-down dbl.              NO     4.  Contains Eudesmol                        →                              YES  Chinook, Hersbruck     .arrow-down dbl.              NO     5.  Cohumulone < 38%                        →                              NO   Cluster, Galena     .arrow-down dbl.              YES     Columbus, Nugget,     Olympic     ______________________________________

Table 9 depicts the key of Peacock and McCarty, which differentiates Aquila, Banner, and Centennial. The data from Appendix 1 is used for all steps except step 4, for which the data in Appendix 2 (which quantifies eusdesmol) is necessary. In Table 9, Step 1 eliminates Aquila and Eroica. Step 2 eliminates the six high β-farnesene hops. Step 3 eliminates Perle, Banner, Hallertau, Liberty, and Mount Hood. Step 4 eliminates Hersbruck and Chinook. Step 5 eliminates Cluster and Galena. Table 7 does not distinguish Columbus from Nugget and Olympic. However, as discussed above, Nugget and Olympic are distinguished from Columbus by each of Table 7 and Table 8.

Finally, the variety Centennial has not yet been distinguished. The data in Appendix 1 show that Centennial has a β-caryophyllene level of 2.649, while Columbus has a β-caryophyllene level of 10.236. Therefore, the varieties are distinguished because they are at virtually opposite ends of the scale for β-caryophyllene.

III. Asexual Reproduction (Propagation) of the Plant

Columbus is asexually reproduced according to methods well known to those having skill in the art. Such methods generally comprise one of three procedures: (a) taking rootstock cuttings known as "roots" or "strap cuts;" (b) converting vine material into woody root-like material in the field and dividing this material into "layered cuttings;" or (c) the use of softwood cuttings, which are also known as "mist cuttings," of green vegetative or shoot material, usually under greenhouse conditions. Further, traditional methods can be applied to make a cell tissue culture from Columbus. Such a cell tissue culture can be stored for extended periods, and gives rise to clones (i.e., asexual progeny) of Columbus. Columbus has been asexually reproduced on ranches associated with HopUnion USA, Inc., located in and about Yakima, Wash. U.S.A.

A. Asexual Reproduction by Taking Rootstock Cuttings

In the field, when main vines die back in the fall, their bases turn woody and swell with the deposition of starch reserves. These bases produce buds at the sites where the main vine nodes were previously located. The bases, usually 4"-8" long and between one-half and two inches thick, are removed by cutting with a sharp knife; other buds on the rootstock produce re-growth in the spring. The detached vine bases are then planted singly or in multiples, in pots or in the field.

Such a procedure typically provides up to a 15-fold increase over the starting number of plants. The increase may be lower where multiple rootstocks (up to 6-8) are to be planted at a single site, to form a single new composite plant.

B. Asexual Reproduction by Layered Cuttings

In the field, main vines are grown up a string, typically to a pre-determined height of about 10 feet. The string is then lowered and the newly grown main vines laid along the ground (for example, for about 8 feet). The string is then re-hung so that a portion of the main vines can renew vertical growth, but in a new location. Some main vines are left laying along the ground and are buried with soil, where the absence of light on the buried, green main vine stimulates it to turn woody, form buds at the nodes and swell with starch deposits. When the unburied portion of the vine has died back, the buried, now woody, portion is dug up and cut up into numerous layered cuttings, which are then replanted to give new plants. Such a procedure provides up to about a 50-fold increase over the starting number of plants.

In an alternative embodiment, new plants are placed at the bottom of deep, open trenches, preferably in a nursery. As the plants grow, soil is periodically pushed into the trench around the vines, thereby yielding layered cuttings in a vertical plane.

C. Asexual Reproduction by Softwood (Mist) Cuttings

Actively growing main vines of a plant are cut into sections comprising a half-node, a single node, or two nodes. Main vines for this purpose are preferably grown in a greenhouse, where growing conditions can be maximized. The cuttings are planted and stuck in a suitable medium, preferably with bottom heat and an intermittent water mist, further preferably under glass. The cuttings produce a rooted plant with new main vine growth about two to three weeks after removal from the original plant. These rooted softwood cuttings can be further grown in pots, in a nursery, or even in the final new plantation. Using such a procedure, it is possible to achieve up to about a 100-fold increase over the starting number of plants.

                  APPENDIX 1     ______________________________________     Detailed GLC analysis of assorted hop varieties.     Analytical Conditions: see Kenny (1990), supra.     OIL AREA PERCENTS OF COMMERCIAL HOPS     ______________________________________     GC/          TENTATIVE IDENTI-     FID  CMPD.   FICATION BY GC./ 21223   21053     RT.  #       MASS SPECTROSCOPY                                   AQUILA  AURORA     ______________________________________     6.42 9       A-PINENE         0.105   0.112     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000   0.326     8.10 16      8-PINENE         0.898   0.817     9.11 18      MYRCENE          62.462  58.731     9.77 22      2-METHYL-BUTYL   0.171   0.159                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.076     9.96 24      2-METHYL-BUTYL   0.370   0.754                  ISOBUTARATE     10.28          26      L-LIMONENE       0.243   0.228     10.62          27      B-PHELLANDRENE?  0.262   0.234                  C10H15     11.59          32      trans-OCIMENE    1.542   0.207     12.45          35      2-METHYL-BUTYL   0.000   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.445   0.656     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.000     14.31          43      METHYL 4-METHYL- 0.069   0.160                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.230   0.264                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.317   0.506     16.26          55      NONANAL          0.048   0.083     19.41          73      METHYL NONANOATE 0.172   0.343     19.56          75      2-DECANONE,      0.139   0.379                  A-YLANGENE     19.93          77      A-COPAENE        0.121   0.161     21.00          83      LINALOOL         0.368   0.997     21.31          88      A-GURJUNENE      0.000   0.000     21.42          89      7-METHYL-2-DECANONE                                   0.062   0.218     22.56          93      C15H24 CALARENE? 0.000   0.000     22.72          95      A-BERGAMOTENE,   0.239   0.408                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.448   1.402     23.10          97      2-DECANOL        0.000   0.160     23.44          99      8-CARYOPHYLLENE  4.104   4.403     23.84          102     METHYL DEC-4-ENOATE                                   1.608   1.286     24.03          103     MW168 2-OL?      0.000   0.181                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   4.371     26.10          122     HUMULENE         1.193   14.141     26.16          123     METHYL DEC-4,8-  1.462   0.310                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.341   0.635                  C15H24     26.59          131     2-DODECANONE     0.311   0.000     27.32          138     A-FARNESENE OR   0.270   0.132                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.000   0.000     27.75          143     A-MUUROLENE      0.114   0.182     27.90          144     B-SELINENE       2.421   0.109     28.04          145     A-SELINENE       2.745   0.130     28.34          147     METHYL           0.266   0.308                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.531   0.696     29.39          155     G-CADINENE       0.191   0.381     30.34          164     CAS# 016728-99-7 OR                                   3.659   0.184                  G-SELINENE     30.73          166     A-MUUROLINE      0.000   0.000     31.15          170     2-TRIDECANONE    1.620   1.080     31.36          171     GERANYL PROPANOATE                                   0.275   0.000     31.46          172     MW 212 METHYL    0.000   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.264   0.000     32.54          179     CAS# 000483-77-2 1.173   0.000                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.109   0.000                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.122   0.282     39.11          211     CARYOPHYLLENE OXIDE                                   0.121   0.083     40.23          221     HUMULENE         0.000   0.530                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.177   0.000     41.07          225     HUMULENE         0.000   0.199                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.598   0.950     43.88          242     MW 220 ME 43,79,91                                   0.170   0.262     44.73          246     T-CADINOL        0.214   0.000     46.08          255     T-MUUROLOL       0.000   0.000                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.407   0.000     ______________________________________     GC/          TENTATIVE IDENTI-     FID  CMPD.   FICATION BY GC./ 21080   21287     RT.  #       MASS SPECTROSCOPY                                   BACKA   BANNER     ______________________________________     6.42 9       A-PINENE         0.000   0.110     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000   0.129     8.10 16      8-PINENE         0.275   0.842     9.11 18      MYRCENE          15.376  65.305     9.77 22      2-METHYL-BUTYL   0.075   0.391                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.173     9.96 24      2-METHYL-BUTYL   0.000   0.988                  ISOBUTARATE     10.28          26      L-LIMONENE       0.108   0.243     10.62          27      B-PHELLANDRENE?  0.000   0.273                  C10H15     11.59          32      trans-OCIMENE    0.000   0.681     12.45          35      2-METHYL-BUTYL   0.000   0.068                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.281   0.381     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.000     14.31          43      METHYL 4-METHYL- 0.298   0.093                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.000   0.413                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.090   0.269     16.26          55      NONANAL          0.939   0.037     19.41          73      METHYL NONANOATE 0.075   0.188     19.56          75      2-DECANONE,      0.497   0.051                  A-YLANGENE     19.93          77      A-COPAENE        0.420   0.173     21.00          83      LINALOOL         0.461   0.583     21.31          88      A-GURJUNENE      0.164   0.166     21.42          89      7-METHYL-2-DECANONE                                   0.384   0.000     22.56          93      C15H24 CALARENE? 0.382   0.069     22.72          95      A-BERGAMOTENE,   0.000   0.243                  METHYL DECANOATE     23.01          96      2-UNDECANONE     1.442   0.046     23.10          97      2-DECANOL        0.685   0.173     23.44          99      8-CARYOPHYLLENE  10.792  5.478     23.84          102     METHYL DEC-4-ENOATE                                   0.588   0.910     24.03          103     MW168 2-OL?      0.394   0.000                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.439   0.000     26.10          122     HUMULENE         37.338  13.256     26.16          123     METHYL DEC-4,8-  0.000   0.157                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   1.342   0.475                  C15H24     26.59          131     2-DODECANONE     0.000   0.000     27.32          138     A-FARNESENE OR   0.114   0.134                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.182   0.094     27.75          143     A-MUUROLENE      0.314   0.173     27.90          144     B-SELINENE       0.391   0.126     28.04          145     A-SELINENE       0.429   0.140     28.34          147     METHYL           0.000   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       1.688   0.873     29.39          155     G-CADINENE       1.075   0.478     30.34          164     CAS# 016728-99-7 OR                                   0.263   0.126                  G-SELINENE     30.73          166     A-MUUROLINE      0.201   0.100     31.15          170     2-TRIDECANONE    0.871   1.945     31.36          171     GERANYL PROPANOATE                                   0.000   0.269     31.46          172     MW 212 METHYL    0.000   0.081                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.000   0.000     32.54          179     CAS# 000483-77-2 0.359   0.082                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.000   0.484                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.236   0.137     39.11          211     CARYOPHYLLENE OXIDE                                   2.753   0.097     40.23          221     HUMULENE         0.173   0.048                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   1.201   0.113     41.07          225     HUMULENE         9.016   0.176                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.124   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   1.910   0.213     43.88          242     MW 220 ME 43,79,91                                   0.500   0.113     44.73          246     T-CADINOL        0.403   0.070     46.08          255     T-MUUROLOL       0.209   0.040                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.333   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.000   0.084     ______________________________________     GC/          TENTATIVE IDENTI-                                   21116     FID  CMPD.   FICATION BY GC./ BREWERS 64100     RT.  #       MASS SPECTROSCOPY                                   GOLD    BULLION     ______________________________________     6.42 9       A-PINENE         0.117   0.110     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.169   0.251     8.10 16      8-PINENE         0.988   0.934     9.11 18      MYRCENE          69.050  67.113     9.77 22      2-METHYL-BUTYL   0.344   0.469                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.068   0.153     9.96 24      2-METHYL-BUTYL   1.267   1.053                  ISOBUTARATE     10.28          26      L-LIMONENE       0.260   0.254     10.62          27      B-PHELLANDRENE?  0.328   0.324                  C10H15     11.59          32      trans-OCIMENE    0.549   0.441     12.45          35      2-METHYL-BUTYL   0.096   0.045                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.298   0.543     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.038     14.31          43      METHYL 4-METHYL- 0.000   0.089                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.105   0.205                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.206   0.174     16.26          55      NONANAL          0.049   0.040     19.41          73      METHYL NONANOATE 0.105   0.208     19.56          75      2-DECANONE,      0.040   0.070                  A-YLANGENE     19.93          77      A-COPAENE        0.166   0.149     21.00          83      LINALOOL         0.461   0.441     21.31          88      A-GURJUNENE      0.039   0.070     21.42          89      7-METHYL-2-DECANONE                                   0.000   0.000     22.56          93      C15H24 CALARENE? 0.085   0.064     22.72          95      A-BERGAMOTENE,   0.105   0.154                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.000   0.119     23.10          97      2-DECANOL        0.179   0.156     23.44          99      8-CARYOPHYLLENE  6.305   6.054     23.84          102     METHYL DEC-4-ENOATE                                   0.566   0.837     24.03          103     MW168 2-OL?      0.000   0.044                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   0.000     26.10          122     HUMULENE         10.326  10.525     26.16          123     METHYL DEC-4,8-  1.153   0.252                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.433   0.396                  C15H24     26.59          131     2-DODECANONE     0.000   0.047     27.32          138     A-FARNESENE OR   0.177   0.169                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.104   0.084     27.75          143     A-MUUROLENE      0.172   0.135     27.90          144     B-SELINENE       0.378   0.433     28.04          145     A-SELINENE       0.410   0.495     28.34          147     METHYL           0.000   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.864   0.790     29.39          155     G-CADINENE       0.443   0.387     30.34          164     CAS# 016728-99-7 OR                                   0.080   0.084                  G-SELINENE     30.73          166     A-MUUROLINE      0.091   0.083     31.15          170     2-TRIDECANONE    0.348   0.802     31.36          171     GERANYL PROPANOATE                                   0.257   0.493     31.46          172     MW 212 METHYL    0.000   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   1.003   0.060     32.54          179     CAS# 000483-77-2 0.000   0.051                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.000   0.126                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.000   0.267     39.11          211     CARYOPHYLLENE OXIDE                                   0.082   0.084     40.23          221     HUMULENE         0.097   0.227                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.047   0.107     41.07          225     HUMULENE         0.115   0.131                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.057   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.073   0.469     43.88          242     MW 220 ME 43,79,91                                   0.000   0.265     44.73          246     T-CADINOL        0.078   0.073     46.08          255     T-MUUROLOL       0.046   0.044                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.586   0.472     ______________________________________     GC/          TENTATIVE IDENTI-        21507     FID  CMPD.   FICATION BY GC./ 56013   CENTE-     RT.  #       MASS SPECTROSCOPY                                   CASCADE NNIAL     ______________________________________     6.42 9       A-PINENE         0.098   0.155     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.110   0.127     8.10 16      8-PINENE         0.933   1.125     9.11 18      MYRCENE          68.051  77.911     9.77 22      2-METHYL-BUTYL   0.260   0.372                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.096   0.234     9.96 24      2-METHYL-BUTYL   0.527   0.911                  ISOBUTARATE     10.28          26      L-LIMONENE       0.233   0.324     10.62          27      B-PHELLANDRENE?  0.337   0.358                  C10H15     11.59          32      trans-OCIMENE    0.132   0.080     12.45          35      2-METHYL-BUTYL   0.000   0.094                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.210   0.333     12.91          40      ISOAMYL ISOVALERATE                                   0.043   0.126     14.31          43      METHYL 4-METHYL- 0.055   0.114                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.091   0.168                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.093   0.193     16.26          55      NONANAL          0.051   0.000     19.41          73      METHYL NONANOATE 0.072   0.101     19.56          75      2-DECANONE,      0.044   0.000                  A-YLANGENE     19.93          77      A-COPAENE        0.118   0.088     21.00          83      LINALOOL         0.312   0.470     21.31          88      A-GURJUNENE      0.000   0.054     21.42          89      7-METHYL-2-DECANONE                                   0.000   0.000     22.56          93      C15H24 CALARENE? 0.070   0.000     22.72          95      A-BERGAMOTENE,   0.275   0.050                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.110   0.000     23.10          97      2-DECANOL        0.175   0.009     23.44          99      8-CARYOPHYLLENE  4.059   4.649     23.84          102     METHYL DEC-4-ENOATE                                   0.333   0.809     24.03          103     MW168 2-OL?      0.000   0.000                  ME 43,81,110,108     25.18          114     B-FARNESENE      5.285   0.000     26.10          122     HUMULENE         10.063  5.699     26.16          123     METHYL DEC-4,8-  0.219   2.229                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.325   0.239                  C15H24     26.59          131     2-DODECANONE     0.000   0.000     27.32          138     A-FARNESENE OR   0.311   0.110                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.087   0.000     27.75          143     A-MUUROLENE      0.107   0.170     27.90          144     B-SELINENE       0.508   0.000     28.04          145     A-SELINENE       0.533   0.050     28.34          147     METHYL           0.195   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.624   0.420     29.39          155     G-CADINENE       0.306   0.246     30.34          164     CAS# 016728-99-7 OR                                   0.071   0.000                  G-SELINENE     30.73          166     A-MUUROLINE      0.066   0.000     31.15          170     2-TRIDECANONE    0.836   0.565     31.36          171     GERANYL PROPANOATE                                   0.402   0.218     31.46          172     MW 212 METHYL    0.109   0.069                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.092   0.762     32.54          179     CAS# 000483-77-2 0.000   0.000                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.047   0.098                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.148   0.000     39.11          211     CARYOPHYLLENE OXIDE                                   0.075   0.072     40.23          221     HUMULENE         0.000   0.000                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.064   0.000     41.07          225     HUMULENE         0.157   0.114                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.490   0.164     43.88          242     MW 220 ME 43,79,91                                   0.382   0.000     44.73          246     T-CADINOL        0.069   0.000     46.08          255     T-MUUROLOL       0.000   0.000                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.093   0.000     ______________________________________     GC/          TENTATIVE IDENTI-                                   21043     FID  CMPD.   FICATION BY GC./ CHALL- 21226     RT.  #       MASS SPECTROSCOPY                                   ANGER  CHINOCK     ______________________________________     6.42 9       A-PINENE         0.078  0.102     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.192  0.270     8.10 16      8-PINENE         0.765  0.768     9.11 18      MYRCENE          55.224 48.105     9.77 22      2-METHYL-BUTYL   0.254  0.477                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.082  0.447     9.96 24      2-METHYL-BUTYL   1.164  2.385                  ISOBUTARATE     10.28          26      L-LIMONENE       0.204  0.210     10.62          27      B-PHELLANDRENE?  0.260  0.228                  C10H15     11.59          32      trans-OCIMENE    0.259  0.067     12.45          35      2-METHYL-BUTYL   0.067  0.185                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.631  0.200     12.91          40      ISOAMYL ISOVALERATE                                   0.000  0.398     14.31          43      METHYL 4-METHYL- 0.337  0.099                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.241  1.211                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.323  0.199     16.26          55      NONANAL          0.076  0.043     19.41          73      METHYL NONANOATE 0.000  0.162     19.56          75      2-DECANONE,      0.189  0.158                  A-YLANGENE     19.93          77      A-COPAENE        0.164  0.530     21.00          83      LINALOOL         0.714  0.400     21.31          88      A-GURJUNENE      0.000  0.696     21.42          89      7-METHYL-2-DECANONE                                   0.076  0.079     22.56          93      C15H24 CALARENE? 0.109  0.186     22.72          95      A-BERGAMOTENE,   0.094  0.156                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.793  0.127     23.10          97      2-DECANOL        0.227  0.453     23.44          99      8-CARYOPHYLLENE  5.394  6.439     23.84          102     METHYL DEC-4-ENOATE                                   0.760  1.180     24.03          103     MW168 2-OL?      0.105  0.044                  ME 43,81,110,108     25.18          114     B-FARNESENE      1.041  0.000     26.10          122     HUMULENE         17.304 14.547     26.16          123     METHYL DEC-4,8-  0.000  0.583                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.232  1.511                  C15H24     26.59          131     2-DODECANONE     0.403  0.000     27.32          138     A-FARNESENE OR   0.319  0.362                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.099  0.331     27.75          143     A-MUUROLENE      0.148  0.510     27.90          144     B-SELINENE       2.769  0.886     28.04          145     A-SELINENE       3.204  0.971     28.34          147     METHYL           0.241  0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.810  2.685     29.39          155     G-CADINENE       0.409  1.493     30.34          164     CAS# 016728-99-7 OR                                   0.105  1.965                  G-SELINENE     30.73          166     A-MUUROLINE      0.000  0.288     31.15          170     2-TRIDECANONE    0.397  0.512     31.36          171     GERANYL PROPANOATE                                   0.000  0.088     31.46          172     MW 212 METHYL    0.000  0.074                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.000  0.979     32.54          179     CAS# 000483-77-2 0.061  0.346                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.000  0.446                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.135  0.089     39.11          211     CARYOPHYLLENE OXIDE                                   0.106  0.066     40.23          221     HUMULENE         0.048  0.131                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.232  0.122     41.07          225     HUMULENE         0.250  0.153                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.063  0.136                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.356  0.268     43.88          242     MW 220 ME 43,79,91                                   0.163  0.000     44.73          246     T-CADINOL        0.077  0.248     46.08          255     T-MUUROLOL       0.057  0.119                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000  0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.076  0.524     ______________________________________     GC/          TENTATIVE IDENTI-                                   65103   65103     FID  CMPD.   FICATION BY GC./ CLUS-   CLUS-     RT.  #       MASS SPECTROSCOPY                                   TER L-1 TER L-2     ______________________________________     6.42 9       A-PINENE         0.103   0.153     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.280   0.153     8.10 16      8-PINENE         0.866   0.655     9.11 18      MYRCENE          60.712  45.653     9.77 22      2-METHYL-BUTYL   0.536   0.335                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.483   0.525     9.96 24      2-METHYL-BUTYL   1.641   1.178                  ISOBUTARATE     10.28          26      L-LIMONENE       0.268   0.235     10.62          27      B-PHELLANDRENE?  0.295   0.210                  C10H15     11.59          32      trans-OCIMENE    0.370   0.229     12.45          35      2-METHYL-BUTYL   0.075   0.070                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.244   0.220     12.91          40      ISOAMYL ISOVALERATE                                   0.187   0.271     14.31          43      METHYL 4-METHYL- 0.342   0.310                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.447   0.399                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.721   0.555     16.26          55      NONANAL          0.250   0.467     19.41          73      METHYL NONANOATE 0.165   0.158     19.56          75      2-DECANONE,      0.060   0.100                  A-YLANGENE     19.93          77      A-COPAENE        0.126   0.242     21.00          83      LINALOOL         0.347   0.259     21.31          88      A-GURJUNENE      0.372   0.374     21.42          89      7-METHYL-2-DECANONE                                   0.071   0.075     22.56          93      C15H24 CALARENE? 0.082   0.158     22.72          95      A-BERGAMOTENE,   0.953   0.855                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.533   0.648     23.10          97      2-DECANOL        0.180   0.352     23.44          99      8-CARYOPHYLLENE  4.725   8.080     23.84          102     METHYL DEC-4-ENOATE                                   1.801   1.927     24.03          103     MW168 2-OL?      0.073   0.108                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   0.000     26.10          122     HUMULENE         13.195  20.805     26.16          123     METHYL DEC-4,8-  0.154   0.132                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.306   0.538                  C15H24     26.59          131     2-DODECANONE     0.064   0.078     27.32          138     A-FARNESENE OR   0.135   0.197                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.084   0.137     27.75          143     A-MUUROLENE      0.127   0.216     27.90          144     B-SELINENE       0.319   0.558     28.04          145     A-SELINENE       0.356   0.612     28.34          147     METHYL           0.000   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.655   1.137     29.39          155     G-CADINENE       0.330   0.604     30.34          164     CAS# 016728-99-7 OR                                   0.081   0.141                  G-SELINENE     30.73          166     A-MUUROLINE      0.059   0.110     31.15          170     2-TRIDECANONE    0.843   1.060     31.36          171     GERANYL PROPANOATE                                   0.000   0.000     31.46          172     MW 212 METHYL    0.741   0.925                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.703   0.446     32.54          179     CAS# 000483-77-2 0.000   0.108                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.228   0.221                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.062   0.082     39.11          211     CARYOPHYLLENE OXIDE                                   0.094   0.251     40.23          221     HUMULENE         0.000   0.147                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.151   0.201     41.07          225     HUMULENE         0.237   0.653                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.137                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.666   1.036     43.88          242     MW 220 ME 43,79,91                                   0.429   0.609     44.73          246     T-CADINOL        0.088   0.170     46.08          255     T-MUUROLOL       0.000   0.090                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.077                  DIEPOXIDE?     48.69          268     FARNESOL         0.066   0.084     ______________________________________     GC/          TENTATIVE IDENTI-     FID  CMPD.   FICATION BY GC./ 21220   21016     RT.  #       MASS SPECTROSCOPY                                   EROICA  FUGGLE     ______________________________________     6.42 9       A-PINENE         0.155   0.075     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000   0.221     8.10 16      8-PINENE         0.884   0.712     9.11 18      MYRCENE          61.773  54.039     9.77 22      2-METHYL-BUTYL   0.237   0.157                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.124   0.000     9.96 24      2-METHYL-BUTYL   0.549   0.443                  ISOBUTARATE     10.28          26      L-LIMONENE       0.233   0.186     10.62          27      B-PHELLANDRENE?  0.264   0.242                  C10H15     11.59          32      trans-OCIMENE    1.577   0.105     12.45          35      2-METHYL-BUTYL   0.000   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.292   0.426     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.000     14.31          43      METHYL 4-METHYL- 0.000   0.170                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.271   0.114                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.257   0.088     16.26          55      NONANAL          0.070   0.098     19.41          73      METHYL NONANOATE 0.204   0.097     19.56          75      2-DECANONE,      0.161   0.212                  A-YLANGENE     19.93          77      A-COPAENE        0.053   0.225     21.00          83      LINALOOL         0.508   0.651     21.31          88      A-GURJUNENE      0.155   0.000     21.42          89      7-METHYL-2-DECANONE                                   0.214   0.083     22.56          93      C15H24 CALARENE? 0.000   0.157     22.72          95      A-BERGAMOTENE,   0.325   0.272                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.820   0.406     23.10          97      2-DECANOL        0.000   0.348     23.44          99      8-CARYOPHYLLENE  12.756  7.628     23.84          102     METHYL DEC-4-ENOATE                                   0.875   0.409     24.03          103     MW168 2-OL?      0.167   0.122                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   4.870     26.10          122     HUMULENE         0.355   20.059     26.16          123     METHYL DEC-4,8-  0.419   0.147                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.104   0.528                  C15H24     26.59          131     2-DODECANONE     0.344   0.073     27.32          138     A-FARNESENE OR   0.000   0.543                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.000   0.090     27.75          143     A-MUUROLENE      0.060   0.173     27.90          144     B-SELINENE       2.268   0.177     28.04          145     A-SELINENE       2.562   0.186     28.34          147     METHYL           0.000   0.424                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.293   1.092     29.39          155     G-CADINENE       0.167   0.573     30.34          164     CAS# 016728-99-7 OR                                   0.000   0.147                  G-SELINENE     30.73          166     A-MUUROLINE      0.000   0.117     31.15          170     2-TRIDECANONE    0.606   0.397     31.36          171     GERANYL PROPANOATE                                   0.091   0.000     31.46          172     MW 212 METHYL    0.000   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.949   0.000     32.54          179     CAS# 000483-77-2 0.077   0.080                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.228   0.064                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.437   0.080     39.11          211     CARYOPHYLLENE OXIDE                                   0.156   0.126     40.23          221     HUMULENE         0.164   0.000                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.878   0.087     41.07          225     HUMULENE         0.000   0.279                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.088                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   1.761   0.435     43.88          242     MW 220 ME 43,79,91                                   0.461   0.235     44.73          246     T-CADINOL        0.055   0.125     46.08          255     T-MUUROLOL       0.000   0.070                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.326   0.134     ______________________________________     GC/          TENTATIVE IDENTI-        21014     FID  CMPD.   FICATION BY GC./ 21102   HALL-     RT.  #       MASS SPECTROSCOPY                                   GALENA  ERTAU     ______________________________________     6.42 9       A-PINENE         0.109   0.064     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.479   0.151     8.10 16      8-PINENE         0.027   0.653     9.11 18      MYRCENE          55.179  53.508     9.77 22      2-METHYL-BUTYL   1.009   0.184                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.554   0.000     9.96 24      2-METHYL-BUTYL   5.004   0.278                  ISOBUTARATE     10.28          26      L-LIMONENE       0.218   0.158     10.62          27      B-PHELLANDRENE?  0.239   0.204                  C10H15     11.59          32      trans-OCIMENE    2.260   0.144     12.45          35      2-METHYL-BUTYL   0.151   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.427   0.647     12.91          40      ISOAMYL ISOVALERATE                                   0.169   0.000     14.31          43      METHYL 4-METHYL- 0.152   0.264                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.930   0.150                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.878   0.203     16.26          55      NONANAL          0.079   0.095     19.41          73      METHYL NONANOATE 0.436   0.138     19.56          75      2-DECANONE,      0.071   0.258                  A-YLANGENE     19.93          77      A-COPAENE        0.111   0.214     21.00          83      LINALOOL         0.206   0.939     21.31          88      A-GURJUNENE      0.577   0.044     21.42          89      7-METHYL-2-DECANONE                                   0.009   0.086     22.56          93      C15H24 CALARENE? 0.053   0.120     22.72          95      A-BERGAMOTENE,   1.317   0.000                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.386   0.696     23.10          97      2-DECANOL        0.131   0.337     23.44          99      8-CARYOPHYLLENE  4.602   7.238     23.84          102     METHYL DEC-4-ENOATE                                   2.237   0.660     24.03          103     MW168 2-OL?      0.042   0.157                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   0.000     26.10          122     HUMULENE         10.599  24.799     26.16          123     METHYL DEC-4,8-  0.180   0.000                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.281   0.670                  C15H24     26.59          131     2-DODECANONE     0.076   0.132     27.32          138     A-FARNESENE OR   0.000   0.227                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.054   0.113     27.75          143     A-MUUROLENE      0.101   0.214     27.90          144     B-SELINENE       0.465   0.190     28.04          145     A-SELINENE       0.544   0.207     28.34          147     METHYL           0.000   0.069                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.586   1.250     29.39          155     G-CADINENE       0.300   0.653     30.34          164     CAS# 016728-99-7 OR                                   0.142   0.151                  G-SELINENE     30.73          166     A-MUUROLINE      0.064   0.136     31.15          170     2-TRIDECANONE    1.561   0.421     31.36          171     GERANYL PROPANOATE                                   0.119   0.000     31.46          172     MW 212 METHYL    0.219   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.000   0.000     32.54          179     CAS# 000483-77-2 0.000   0.102                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      1.725   0.071                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.186   0.125     39.11          211     CARYOPHYLLENE OXIDE                                   0.064   0.111     40.23          221     HUMULENE         0.000   0.099                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.237   0.239     41.07          225     HUMULENE         0.134   0.357                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.100                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.579   0.638     43.88          242     MW 220 ME 43,79,91                                   0.263   0.263     44.73          246     T-CADINOL        0.059   0.125     46.08          255     T-MUUROLOL       0.000   0.075                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.000   0.064     ______________________________________     GC/          TENTATIVE IDENTI-                                   21135  21097     FID  CMPD.   FICATION BY GC./ HERS-  HULLERER     RT.  #       MASS SPECTROSCOPY                                   BRUCK  BITTERER     ______________________________________     6.42 9       A-PINENE         0.000  0.087     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000  0.088     8.10 16      8-PINENE         0.527  0.737     9.11 18      MYRCENE          44.828 52.818     9.77 22      2-METHYL-BUTYL   0.000  0.415                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000  0.168     9.96 24      2-METHYL-BUTYL   0.066  0.844                  ISOBUTARATE     10.28          26      L-LIMONENE       0.147  0.207     10.62          27      B-PHELLANDRENE?  0.145  0.212                  C10H15     11.59          32      trans-OCIMENE    0.000  0.092     12.45          35      2-METHYL-BUTYL   0.000  0.079                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.418  0.231     12.91          40      ISOAMYL ISOVALERATE                                   0.000  0.075     14.31          43      METHYL 4-METHYL- 0.289  0.000                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.171  0.408                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.134  0.110     16.26          55      NONANAL          0.226  0.084     19.41          73      METHYL NONANOATE 0.090  0.088     19.56          75      2-DECANONE,      0.334  0.251                  A-YLANGENE     19.93          77      A-COPAENE        0.259  0.617     21.00          83      LINALOOL         0.805  0.503     21.31          88      A-GURJUNENE      0.077  0.169     21.42          89      7-METHYL-2-DECANONE                                   0.214  0.121     22.56          93      C15H24 CALARENE? 0.190  0.300     22.72          95      A-BERGAMOTENE,   0.000  0.000                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.921  0.387     23.10          97      2-DECANOL        0.531  0.602     23.44          99      8-CARYOPHYLLENE  5.573  4.386     23.84          102     METHYL DEC-4-ENOATE                                   0.551  0.772     24.03          103     MW168 2-OL?      0.210  0.122                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.324  0.093     26.10          122     HUMULENE         29.352 8.090     26.16          123     METHYL DEC-4,8-  0.132  0.352                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.735  1.994                  C15H24     26.59          131     2-DODECANONE     0.203  0.000     27.32          138     A-FARNESENE OR   0.326  0.273                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.103  0.352     27.75          143     A-MUUROLENE      0.248  0.574     27.90          144     B-SELINENE       0.306  2.221     28.04          145     A-SELINENE       0.335  2.426     28.34          147     METHYL           0.191  0.399                  ?-UNDECENOATE     29.16          154     D-CADINENE       1.330  2.714     29.39          155     G-CADINENE       0.746  1.516     30.34          164     CAS# 016728-99-7 OR                                   0.141  3.993                  G-SELINENE     30.73          166     A-MUUROLINE      0.146  0.364     31.15          170     2-TRIDECANONE    0.580  0.241     31.36          171     GERANYL PROPANOATE                                   0.000  0.000     31.46          172     MW 212 METHYL    0.000  0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.000  0.134     32.54          179     CAS# 000483-77-2 0.229  0.205                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.000  0.340                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.147  0.179     39.11          211     CARYOPHYLLENE OXIDE                                   0.331  0.082     40.23          221     HUMULENE         0.145  0.137                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.260  0.000     41.07          225     HUMULENE         0.962  0.194                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.159  0.074                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.752  0.531     43.88          242     MW 220 ME 43,79,91                                   0.489  0.195     44.73          246     T-CADINOL        0.177  0.336     46.08          255     T-MUUROLOL       0.114  0.132                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000  0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.161  0.166     ______________________________________     GC/          TENTATIVE IDENTI-        21113     FID  CMPD.   FICATION BY GC./ 21457   LUB-     RT.  #       MASS SPECTROSCOPY                                   LIBERTY ELSKA     ______________________________________     6.42 9       A-PINENE         0.043   0.047     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.068   0.000     8.10 16      8-PINENE         0.504   0.421     9.11 18      MYRCENE          41.506  29.558     9.77 22      2-METHYL-BUTYL   0.117   0.000                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.000     9.96 24      2-METHYL-BUTYL   0.197   0.176                  ISOBUTARATE     10.28          26      L-LIMONENE       0.124   0.145     10.62          27      B-PHELLANDRENE?  0.149   0.099                  C10H15     11.59          32      trans-OCIMENE    0.066   0.000     12.45          35      2-METHYL-BUTYL   0.000   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.293   0.259     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.000     14.31          43      METHYL 4-METHYL- 0.128   0.081                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.083   0.148                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.189   0.100     16.26          55      NONANAL          0.091   0.193     19.41          73      METHYL NONANOATE 0.092   0.183     19.56          75      2-DECANONE,      0.240   0.268                  A-YLANGENE     19.93          77      A-COPAENE        0.304   0.268     21.00          83      LINALOOL         0.814   0.254     21.31          88      A-GURJUNENE      0.043   0.106     21.42          89      7-METHYL-2-DECANONE                                   0.098   0.198     22.56          93      C15H24 CALARENE? 0.172   0.179     22.72          95      A-BERGAMOTENE,   0.000   0.947                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.737   0.690     23.10          97      2-DECANOL        0.442   0.443     23.44          99      8-CARYOPHYLLENE  9.651   6.953     23.84          102     METHYL DEC-4-ENOATE                                   0.663   1.089     24.03          103     MW168 2-OL?      0.140   0.207                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.080   17.712     26.10          122     HUMULENE         32.402  21.404     26.16          123     METHYL DEC-4,8-  0.075   0.451                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.387   0.796                  C15H24     26.59          131     2-DODECANONE     0.105   0.167     27.32          138     A-FARNESENE OR   0.509   0.316                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.181   0.157     27.75          143     A-MUUROLENE      0.289   0.282     27.90          144     B-SELINENE       0.325   1.492     28.04          145     A-SELINENE       0.358   1.546     28.34          147     METHYL           0.310   0.242                  ?-UNDECENOATE     29.16          154     D-CADINENE       1.508   1.438     29.39          155     G-CADINENE       0.887   0.811     30.34          164     CAS# 016728-99-7 OR                                   0.213   0.237                  G-SELINENE     30.73          166     A-MUUROLINE      0.103   0.159     31.15          170     2-TRIDECANONE    0.458   0.738     31.36          171     GERANYL PROPANOATE                                   0.000   0.000     31.46          172     MW 212 METHYL    0.000   0.070                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.099   0.257     32.54          179     CAS# 000483-77-2 0.193   0.196                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.057   0.203                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.115   0.199     39.11          211     CARYOPHYLLENE OXIDE                                   0.205   0.550     40.23          221     HUMULENE         0.103   0.298                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.308   0.261     41.07          225     HUMULENE         0.599   1.612                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.144   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.568   1.358     43.88          242     MW 220 ME 43,79,91                                   0.342   0.439     44.73          246     T-CADINOL        0.179   0.247     46.08          255     T-MUUROLOL       0.128   0.131                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.094                  DIEPOXIDE?     48.69          268     FARNESOL         0.340   0.339     ______________________________________                                           21073     GC/          TENTATIVE IDENTI-                                   21114   NORTH-     FID  CMPD.   FICATION BY GC./ NADWIS- ERN     RT.  #       MASS SPECTROSCOPY                                   LANSKA  BREWER     ______________________________________     6.42 9       A-PINENE         0.066   0.125     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000   0.174     8.10 16      8-PINENE         0.649   0.862     9.11 18      MYRCENE          44.179  55.587     9.77 22      2-METHYL-BUTYL   0.000   0.183                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.108     9.96 24      2-METHYL-BUTYL   0.000   0.428                  ISOBUTARATE     10.28          26      L-LIMONENE       0.169   0.248     10.62          27      B-PHELLANDRENE?  0.149   0.265                  C10H15     11.59          32      trans-OCIMENE    0.000   0.488     12.45          35      2-METHYL-BUTYL   0.000   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.391   0.706     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.000     14.31          43      METHYL 4-METHYL- 0.206   0.251                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.178   0.150                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.113   0.199     16.26          55      NONANAL          0.141   0.077     19.41          73      METHYL NONANOATE 0.155   0.166     19.56          75      2-DECANONE,      0.260   0.177                  A-YLANGENE     19.93          77      A-COPAENE        0.160   0.284     21.00          83      LINALOOL         0.390   0.344     21.31          88      A-GURJUNENE      0.073   0.000     21.42          89      7-METHYL-2-DECANONE                                   0.116   0.049     22.56          93      C15H24 CALARENE? 0.113   0.154     22.72          95      A-BERGAMOTENE,   0.854   0.000                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.592   0.292     23.10          97      2-DECANOL        0.284   0.307     23.44          99      8-CARYOPHYLLENE  5.415   9.217     23.84          102     METHYL DEC-4-ENOATE                                   0.939   0.565     24.03          103     MW168 2-OL?      0.211   0.077                  ME 43,81,110,108     25.18          114     B-FARNESENE      17.417  0.000     26.10          122     HUMULENE         18.740  21.642     26.16          123     METHYL DEC-4,8-  0.368   0.389                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.461   0.619                  C15H24     26.59          131     2-DODECANONE     0.127   0.084     27.32          138     A-FARNESENE OR   0.264   0.179                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.053   0.112     27.75          143     A-MUUROLENE      0.143   0.217     27.90          144     B-SELINENE       0.217   0.182     28.04          145     A-SELINENE       0.161   0.210     28.34          147     METHYL           0.206   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.766   1.301     29.39          155     G-CADINENE       0.431   0.650     30.34          164     CAS# 016728-99-7 OR                                   0.121   0.129                  G-SELINENE     30.73          166     A-MUUROLINE      0.085   0.124     31.15          170     2-TRIDECANONE    0.417   0.337     31.36          171     GERANYL PROPANOATE                                   0.000   0.000     31.46          172     MW 212 METHYL    0.000   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.200   0.000     32.54          179     CAS# 000483-77-2 0.131   0.110                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.146   0.068                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.127   0.082     39.11          211     CARYOPHYLLENE OXIDE                                   0.284   0.122     40.23          221     HUMULENE         0.156   0.065                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.127   0.109     41.07          225     HUMULENE         0.855   0.250                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.112   0.090                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.755   0.295     43.88          242     MW 220 ME 43,79,91                                   0.210   0.169     44.73          246     T-CADINOL        0.113   0.119     46.08          255     T-MUUROLOL       0.045   0.057                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.187   0.230     ______________________________________     GC/          TENTATIVE IDENTI-                                   21044     FID  CMPD.   FICATION BY GC./ NORTH-  21197     RT.  #       MASS SPECTROSCOPY                                   DOWN    NUGGET     ______________________________________     6.42 9       A-PINENE         0.066   0.095     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000   0.244     8.10 16      8-PINENE         0.639   0.671     9.11 18      MYRCENE          42.280  60.676     9.77 22      2-METHYL-BUTYL   0.106   0.559                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.550     9.96 24      2-METHYL-BUTYL   0.173   1.307                  ISOBUTARATE     10.28          26      L-LIMONENE       0.173   0.203     10.62          27      B-PHELLANDRENE?  0.199   0.244                  C10H15     11.59          32      trans-OCIMENE    0.104   0.571     12.45          35      2-METHYL-BUTYL   0.000   0.063                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.337   0.615     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.124     14.31          43      METHYL 4-METHYL- 0.127   0.201                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.098   0.257                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.165   0.470     16.26          55      NONANAL          0.000   0.052     19.41          73      METHYL NONANOATE 0.122   0.326     19.56          75      2-DECANONE,      0.000   0.052                  A-YLANGENE     19.93          77      A-COPAENE        0.321   0.167     21.00          83      LINALOOL         0.559   0.904     21.31          88      A-GURJUNENE      0.000   0.113     21.42          89      7-METHYL-2-DECANONE                                   0.000   0.000     22.56          93      C15H24 CALARENE? 0.228   0.069     22.72          95      A-BERGAMOTENE,   0.000   0.298                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.181   0.363     23.10          97      2-DECANOL        0.474   0.230     23.44          99      8-CARYOPHYLLENE  12.260  6.289     23.84          102     METHYL DEC-4-ENOATE                                   0.798   1.366     24.03          103     MW168 2-OL?      0.000   0.054                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.746   0.000     26.10          122     HUMULENE         31.313  15.386     26.16          123     METHYL DEC-4,8-  0.000   0.128                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.198   0.359                  C15H24     26.59          131     2-DODECANONE     0.800   0.077     27.32          138     A-FARNESENE OR   0.210   0.321                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.153   0.086     27.75          143     A-MUUROLENE      0.300   0.237     27.90          144     B-SELINENE       0.291   0.560     28.04          145     A-SELINENE       0.324   0.647     28.34          147     METHYL           0.000   0.164                  ?-UNDECENOATE     29.16          154     D-CADINENE       1.517   0.760     29.39          155     G-CADINENE       0.815   0.399     30.34          164     CAS# 016728-99-7 OR                                   0.146   0.102                  G-SELINENE     30.73          166     A-MUUROLINE      0.163   0.075     31.15          170     2-TRIDECANONE    0.149   0.500     31.36          171     GERANYL PROPANOATE                                   0.000   0.000     31.46          172     MW 212 METHYL    0.000   0.077                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.000   0.000     32.54          179     CAS# 000483-77-2 0.199   0.000                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.000   0.119                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.000   0.112     39.11          211     CARYOPHYLLENE OXIDE                                   0.410   0.051     40.23          221     HUMULENE         0.089   0.000                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.067   0.328     41.07          225     HUMULENE         0.801   0.113                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.153   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.168   0.643     43.88          242     MW 220 ME 43,79,91                                   0.101   0.206     44.73          246     T-CADINOL        0.138   0.062     46.08          255     T-MUUROLOL       0.086   0.000                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.154   0.071     ______________________________________     GC/          TENTATIVE IDENTI-     FID  CMPD.   FICATION BY GC./ 21223   21227     RT.  #       MASS SPECTROSCOPY                                   OLYMPIC PERLE     ______________________________________     6.42 9       A-PINENE         0.127   0.077     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.116   0.193     8.10 16      8-PINENE         1.037   0.618     9.11 18      MYRCENE          61.466  41.489     9.77 22      2-METHYL-BUTYL   0.470   0.120                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.121   0.000     9.96 24      2-METHYL-BUTYL   1.424   0.308                  ISOBUTARATE     10.28          26      L-LIMONENE       0.247   0.177     10.62          27      B-PHELLANDRENE?  0.269   0.199                  C10H15     11.59          32      trans-OCIMENE    0.040   0.591     12.45          35      2-METHYL-BUTYL   0.549   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.136   0.425     12.91          40      ISOAMYL ISOVALERATE                                   0.476   0.000     14.31          43      METHYL 4-METHYL- 0.051   0.111                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.216   0.061                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.066   0.053     16.26          55      NONANAL          0.053   0.096     19.41          73      METHYL NONANOATE 0.040   0.047     19.56          75      2-DECANONE,      0.056   0.256                  A-YLANGENE     19.93          77      A-COPAENE        0.201   0.350     21.00          83      LINALOOL         0.316   0.242     21.31          88      A-GURJUNENE      0.096   0.000     21.42          89      7-METHYL-2-DECANONE                                   0.000   0.047     22.56          93      C15H24 CALARENE? 0.093   0.224     22.72          95      A-BERGAMOTENE,   0.055   0.000                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.000   0.319     23.10          97      2-DECANOL        0.238   0.487     23.44          99      8-CARYOPHYLLENE  8.722   12.480     23.84          102     METHYL DEC-4-ENOATE                                   0.743   0.150     24.03          103     MW168 2-OL?      0.000   0.096                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   0.162     26.10          122     HUMULENE         13.614  31.397     26.16          123     METHYL DEC-4,8-  0.408   0.175                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.518   0.845                  C15H24     26.59          131     2-DODECANONE     0.000   0.110     27.32          138     A-FARNESENE OR   0.177   0.296                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.116   0.183     27.75          143     A-MUUROLENE      0.248   0.315     27.90          144     B-SELINENE       0.738   0.276     28.04          145     A-SELINENE       0.764   0.320     28.34          147     METHYL           0.000   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.979   1.770     29.39          155     G-CADINENE       0.547   0.862     30.34          164     CAS# 016728-99-7 OR                                   0.116   0.190                  G-SELINENE     30.73          166     A-MUUROLINE      0.107   0.177     31.15          170     2-TRIDECANONE    0.300   0.273     31.36          171     GERANYL PROPANOATE                                   0.105   0.000     31.46          172     MW 212 METHYL    0.065   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   1.055   0.000     32.54          179     CAS# 000483-77-2 0.084   0.123                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.063   0.000                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.000   0.077     39.11          211     CARYOPHYLLENE OXIDE                                   0.166   0.152     40.23          221     HUMULENE         0.059   0.072                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.113   0.100     41.07          225     HUMULENE         0.224   0.366                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.076   0.136                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.253   0.389     43.88          242     MW 220 ME 43,79,91                                   0.139   0.276     44.73          246     T-CADINOL        0.097   0.186     46.08          255     T-MUUROLOL       0.057   0.115                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.127   0.192     ______________________________________     GC/          TENTATIVE IDENTI-                                   60052     FID  CMPD.   FICATION BY GC./ PRIDE OF 21077     RT.  #       MASS SPECTROSCOPY                                   RINGWOOD SAAZ     ______________________________________     6.42 9       A-PINENE         0.089    0.049     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.043    0.000     8.10 16      8-PINENE         0.690    0.486     9.11 18      MYRCENE          46.727   34.050     9.77 22      2-METHYL-BUTYL   0.121    0.000                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000    0.000     9.96 24      2-METHYL-BUTYL   0.220    0.000                  ISOBUTARATE     10.28          26      L-LIMONENE       0.197    0.166     10.62          27      B-PHELLANDRENE?  0.156    0.081                  C10H15     11.59          32      trans-OCIMENE    0.000    0.000     12.45          35      2-METHYL-BUTYL   0.000    0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.334    0.626     12.91          40      ISOAMYL ISOVALERATE                                   0.000    0.000     14.31          43      METHYL 4-METHYL- 0.180    0.258                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.319    0.340                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.473    0.225     16.26          55      NONANAL          0.116    0.308     19.41          73      METHYL NONANOATE 0.250    0.380     19.56          75      2-DECANONE,      0.147    0.501                  A-YLANGENE     19.93          77      A-COPAENE        0.241    0.191     21.00          83      LINALOOL         0.317    0.337     21.31          88      A-GURJUNENE      0.230    0.173     21.42          89      7-METHYL-2-DECANONE                                   0.125    0.222     22.56          93      C15H24 CALARENE? 0.133    0.113     22.72          95      A-BERGAMOTENE,   0.326    1.043                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.690    1.394     23.10          97      2-DECANOL        0.226    0.299     23.44          99      8-CARYOPHYLLENE  8.708    5.010     23.84          102     METHYL DEC-4-ENOATE                                   1.808    1.769     24.03          103     MW168 2-OL?      0.230    0.417                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000    18.900     26.10          122     HUMULENE         1.946    18.111     26.16          123     METHYL DEC-4,8-  0.571    0.259                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.680    0.531                  C15H24     26.59          131     2-DODECANONE     0.053    0.264     27.32          138     A-FARNESENE OR   1.932    0.255                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.000    0.134     27.75          143     A-MUUROLENE      0.000    0.189     27.90          144     B-SELINENE       9.372    0.255     28.04          145     A-SELINENE       10.128   0.178     28.34          147     METHYL           1.329    0.104                  ?-UNDECENOATE     29.16          154     D-CADINENE       1.124    0.829     29.39          155     G-CADINENE       0.650    0.519     30.34          164     CAS# 016728-99-7 OR                                   0.137    0.181                  G-SELINENE     30.73          166     A-MUUROLINE      0.133    0.099     31.15          170     2-TRIDECANONE    0.551    0.957     31.36          171     GERANYL PROPANOATE                                   0.000    0.000     31.46          172     MW 212 METHYL    0.104    0.081                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.217    0.080     32.54          179     CAS# 000483-77-2 0.146    0.167                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.182    0.424                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.079    0.272     39.11          211     CARYOPHYLLENE OXIDE                                   0.337    0.476     40.23          221     HUMULENE         0.076    0.329                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.175    0.294     41.07          225     HUMULENE         0.106    1.530                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.082    0.155                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.852    1.789     43.88          242     MW 220 ME 43,79,91                                   0.231    0.487     44.73          246     T-CADINOL        0.140    0.174     46.08          255     T-MUUROLOL       0.067    0.099                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000    0.083                  DIEPOXIDE?     48.69          268     FARNESOL         0.169    0.160     ______________________________________     GC/          TENTATIVE IDENTI-        21173     FID  CMPD.   FICATION BY GC./ 21186   STRISS-     RT.  #       MASS SPECTROSCOPY                                   SPALTER LESPALT     ______________________________________     6.42 9       A-PINENE         0.091   0.062     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000   0.202     8.10 16      8-PINENE         0.861   0.634     9.11 18      MYRCENE          60.356  43.977     9.77 22      2-METHYL-BUTYL   0.000   0.257                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.112     9.96 24      2-METHYL-BUTYL   0.000   0.736                  ISOBUTARATE     10.28          26      L-LIMONENE       0.225   0.175     10.62          27      B-PHELLANDRENE?  0.234   0.139                  C10H15     11.59          32      trans-OCIMENE    0.055   0.082     12.45          35      2-METHYL-BUTYL   0.000   0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.658   0.326     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.000     14.31          43      METHYL 4-METHYL- 0.238   0.323                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.230   0.000                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.168   0.103     16.26          55      NONANAL          0.166   0.255     19.41          73      METHYL NONANOATE 0.341   0.119     19.56          75      2-DECANONE,      0.313   0.216                  A-YLANGENE     19.93          77      A-COPAENE        0.090   0.293     21.00          83      LINALOOL         0.557   0.649     21.31          88      A-GURJUNENE      0.127   0.000     21.42          89      7-METHYL-2-DECANONE                                   0.144   0.133     22.56          93      C15H24 CALARENE? 0.055   0.243     22.72          95      A-BERGAMOTENE,   0.691   0.000                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.612   0.385     23.10          97      2-DECANOL        0.179   0.509     23.44          99      8-CARYOPHYLLENE  2.481   10.500     23.84          102     METHYL DEC-4-ENOATE                                   1.180   0.570     24.03          103     MW168 2-OL?      0.211   0.091                  ME 43,81,110,108     25.18          114     B-FARNESENE      14.019  0.000     26.10          122     HUMULENE         9.083   29.252     26.16          123     METHYL DEC-4,8-  0.313   0.147                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.243   0.872                  C15H24     26.59          131     2-DODECANONE     0.174   0.000     27.32          138     A-FARNESENE OR   0.179   0.131                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.000   0.088     27.75          143     A-MUUROLENE      0.137   0.233     27.90          144     B-SELINENE       0.130   0.237     28.04          145     A-SELINENE       0.069   0.281     28.34          147     METHYL           0.118   0.082                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.398   1.364     29.39          155     G-CADINENE       0.226   0.824     30.34          164     CAS# 016728-99-7 OR                                   0.119   0.210                  G-SELINENE     30.73          166     A-MUUROLINE      0.000   0.196     31.15          170     2-TRIDECANONE    0.479   0.255     31.36          171     GERANYL PROPANOATE                                   0.000   0.000     31.46          172     MW 212 METHYL    0.063   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.193   0.000     32.54          179     CAS# 000483-77-2 0.073   0.205                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.233   0.000                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.155   0.172     39.11          211     CARYOPHYLLENE OXIDE                                   0.139   0.540     40.23          221     HUMULENE         0.106   0.152                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.137   0.150     41.07          225     HUMULENE         0.425   1.140                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.854   0.609     43.88          242     MW 220 ME 43,79,91                                   0.186   0.141     44.73          246     T-CADINOL        0.064   0.144     46.08          255     T-MUUROLOL       0.000   0.092                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.093   0.191     ______________________________________     GC/          TENTATIVE IDENTI-     FID  CMPD.   FICATION BY GC./ 21049   21112     RT.  #       MASS SPECTROSCOPY                                   STYRIAN TARGET     ______________________________________     6.42 9       A-PINENE         0.089   0.119     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.328   0.406     8.10 16      8-PINENE         0.795   0.865     9.11 18      MYRCENE          56.296  58.164     9.77 22      2-METHYL-BUTYL   0.171   0.482                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000   0.461     9.96 24      2-METHYL-BUTYL   0.753   1.408                  ISOBUTARATE     10.28          26      L-LIMONENE       0.207   0.228     10.62          27      B-PHELLANDRENE?  0.229   0.226                  C10H15     11.59          32      trans-OCIMENE    0.057   0.366     12.45          35      2-METHYL-BUTYL   0.000   0.054                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.270   0.602     12.91          40      ISOAMYL ISOVALERATE                                   0.000   0.118     14.31          43      METHYL 4-METHYL- 0.305   0.427                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.000   0.589                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.000   0.446     16.26          55      NONANAL          0.141   0.107     19.41          73      METHYL NONANOATE 0.070   0.689     19.56          75      2-DECANONE,      0.216   0.821                  A-YLANGENE     19.93          77      A-COPAENE        0.198   0.336     21.00          83      LINALOOL         0.751   0.891     21.31          88      A-GURJUNENE      0.000   0.000     21.42          89      7-METHYL-2-DECANONE                                   0.094   0.401     22.56          93      C15H24 CALARENE? 0.166   0.000     22.72          95      A-BERGAMOTENE,   0.266   0.519                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.301   1.502     23.10          97      2-DECANOL        0.366   0.282     23.44          99      8-CARYOPHYLLENE  7.269   4.952     23.84          102     METHYL DEC-4-ENOATE                                   0.196   1.912     24.03          103     MW168 2-OL?      0.096   0.214                  ME 43,81,110,108     25.18          114     B-FARNESENE      4.187   0.000     26.10          122     HUMULENE         20.607  10.291     26.16          123     METHYL DEC-4,8-  0.106   0.221                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.527   1.123                  C15H24     26.59          131     2-DODECANONE     0.000   0.000     27.32          138     A-FARNESENE OR   0.000   0.233                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.000   0.165     27.75          143     A-MUUROLENE      0.211   0.215     27.90          144     B-SELINENE       0.152   0.329     28.04          145     A-SELINENE       0.184   0.404     28.34          147     METHYL           0.269   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.013   1.215     29.39          155     G-CADINENE       0.536   0.653     30.34          164     CAS# 016728-99-7 OR                                   0.000   0.798                  G-SELINENE     30.73          166     A-MUUROLINE      0.167   0.198     31.15          170     2-TRIDECANONE    0.228   0.974     31.36          171     GERANYL PROPANOATE                                   0.000   0.000     31.46          172     MW 212 METHYL    0.000   0.114                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.000   0.000     32.54          179     CAS# 000483-77-2 0.112   0.118                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.000   0.388                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.000   0.460     39.11          211     CARYOPHYLLENE OXIDE                                   0.265   0.095     40.23          221     HUMULENE         0.000   0.536                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.000   0.000     41.07          225     HUMULENE         0.569   0.210                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.000   0.000                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.394   1.253     43.88          242     MW 220 ME 43,79,91                                   0.119   0.266     44.73          246     T-CADINOL        0.119   0.114     46.08          255     T-MUUROLOL       0.000   0.000                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.000   0.000     ______________________________________     GC/          TENTATIVE IDENTI-                                   21015  21041     FID  CMPD.   FICATION BY GC./ TETT-  WTILAM-     RT.  #       MASS SPECTROSCOPY                                   NANG   EITF     ______________________________________     6.42 9       A-PINENE         0.080  0.078     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.000  0.217     8.10 16      8-PINENE         0.753  0.741     9.11 18      MYRCENE          50.137 55.492     9.77 22      2-METHYL-BUTYL   0.000  0.234                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.000  0.000     9.96 24      2-METHYL-BUTYL   0.000  0.507                  ISOBUTARATE     10.28          26      L-LIMONENE       0.205  0.191     10.62          27      B-PHELLANDRENE?  0.147  0.251                  C10H15     11.59          32      trans-OCIMENE    0.000  0.114     12.45          35      2-METHYL-BUTYL   0.000  0.000                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.509  0.532     12.91          40      ISOAMYL ISOVALERATE                                   0.000  0.000     14.31          43      METHYL 4-METHYL- 0.230  0.224                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.260  0.120                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.172  0.087     16.26          55      NONANAL          0.233  0.073     19.41          73      METHYL NONANOATE 0.228  0.078     19.56          75      2-DECANONE,      0.290  0.097                  A-YLANGENE     19.93          77      A-COPAENE        0.129  0.190     21.00          83      LINALOOL         0.441  0.661     21.31          88      A-GURJUNENE      0.125  0.000     21.42          89      7-METHYL-2-DECANONE                                   0.199  0.000     22.56          93      C15H24 CALARENE? 0.085  0.141     22.72          95      A-BERGAMOTENE,   0.873  0.380                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.759  0.131     23.10          97      2-DECANOL        0.257  0.313     23.44          99      8-CARYOPHYLLENE  3.697  6.934     23.84          102     METHYL DEC-4-ENOATE                                   1.220  0.288     24.03          103     MW168 2-OL?      0.255  0.041                  ME 43,81,110,108     25.18          114     B-FARNESENE      16.938 7.097     26.10          122     HUMULENE         13.277 18.815     26.16          123     METHYL DEC-4,8-  0.338  0.196                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.321  0.472                  C15H24     26.59          131     2-DODECANONE     0.169  0.000     27.32          138     A-FARNESENE OR   0.194  0.599                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.000  0.106     27.75          143     A-MUUROLENE      0.121  0.170     27.90          144     B-SELINENE       0.166  0.245     28.04          145     A-SELINENE       0.099  0.238     28.34          147     METHYL           0.177  0.407                  ?-UNDECENOATE     29.16          154     D-CADINENE       0.532  0.970     29.39          155     G-CADINENE       0.317  0.493     30.34          164     CAS# 016728-99-7 OR                                   0.114  0.107                  G-SELINENE     30.73          166     A-MUUROLINE      0.059  0.099     31.15          170     2-TRIDECANONE    0.574  0.142     31.36          171     GERANYL PROPANOATE                                   0.000  0.000     31.46          172     MW 212 METHYL    0.000  0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.157  0.050     32.54          179     CAS# 000483-77-2 0.131  0.071                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.192  0.000                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.163  0.000     39.11          211     CARYOPHYLLENE OXIDE                                   0.282  0.097     40.23          221     HUMULENE         0.180  0.000                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.161  0.000     41.07          225     HUMULENE         0.901  0.200                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.089  0.071                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   1.033  0.118     43.88          242     MW 220 ME 43,79,91                                   0.235  0.000     44.73          246     T-CADINOL        0.098  0.104     46.08          255     T-MUUROLOL       0.000  0.061                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000  0.000                  DIEPOXIDE?     48.69          268     FARNESOL         0.131  0.457     ______________________________________     GC/          TENTATIVE IDENTI-        9/17     FID  CMPD.   FICATION BY GC./ 21498   COLUM-     RT.  #       MASS SPECTROSCOPY                                   YEOMAN  BUS     ______________________________________     6.42 9       A-PINENE         0.121   0.125     7.46 14      ISOBUTYL ISOBUTYRATE                                   0.455   0.066     8.10 16      8-PINENE         0.829   0.745     9.11 18      MYRCENE          51.427  42.267     9.77 22      2-METHYL-BUTYL   0.659   0.370                  PROPANOATE     9.90 23      ISOAMYL ISOBUTYRATE                                   0.522   0.184     9.96 24      2-METHYL-BUTYL   1.946   0.800                  ISOBUTARATE     10.28          26      L-LIMONENE       0.237   0.236     10.62          27      B-PHELLANDRENE?  0.260   0.177                  C10H15     11.59          32      trans-OCIMENE    0.159   0.168     12.45          35      2-METHYL-BUTYL   0.180   0.090                  2-METHYL-BUTYRATE     12.68          36      METHYL HEPTANOATE                                   0.349   0.000     12.91          40      ISOAMYL ISOVALERATE                                   0.274   0.106     14.31          43      METHYL 4-METHYL- 0.139   0.000                  HEXA-2-ENOATE     14.39          45      METHYL 6-METHYL- 0.119   0.153                  HEPTANOATE     15.98          51      METHYL OCTANOATE 0.209   0.000     16.26          55      NONANAL          0.059   0.000     19.41          73      METHYL NONANOATE 0.070   0.000     19.56          75      2-DECANONE,      0.133                  A-YLANGENE     19.93          77      A-COPAENE        0.252   0.764     21.00          83      LINALOOL         0.379   0.417     21.31          88      A-GURJUNENE      0.000     21.42          89      7-METHYL-2-DECANONE                                   0.000   0.000     22.56          93      C15H24 CALARENE? 0.123   0.252     22.72          95      A-BERGAMOTENE,   0.000   0.000                  METHYL DECANOATE     23.01          96      2-UNDECANONE     0.361   0.068     23.10          97      2-DECANOL        0.268     23.44          99      8-CARYOPHYLLENE  6.954   10.236     23.84          102     METHYL DEC-4-ENOATE                                   0.692   0.457     24.03          103     MW168 2-OL?      0.115   0.000                  ME 43,81,110,108     25.18          114     B-FARNESENE      0.000   0.000     26.10          122     HUMULENE         10.146  17.370     26.16          123     METHYL DEC-4,8-  0.553                  DIENOATE     26.46          129     WILEY# 123998 MW 204                                   0.439   1.992                  C15H24     26.59          131     2-DODECANONE     0.045   0.000     27.32          138     A-FARNESENE OR   0.223   0.508                  ZINGIBERENE     27.53          139     CAS# 030021-74-0 0.117   0.464     27.75          143     A-MUUROLENE      0.209   0.733     27.90          144     B-SELINENE       2.829   1.230     28.04          145     A-SELINENE       3.352   1.291     28.34          147     METHYL           0.075   0.000                  ?-UNDECENOATE     29.16          154     D-CADINENE       1.028   3.412     29.39          155     G-CADINENE       0.484   2.048     30.34          164     CAS# 016728-99-7 OR                                   0.134   2.809                  G-SELINENE     30.73          166     A-MUUROLINE      0.095   0.366     31.15          170     2-TRIDECANONE    0.313   0.000     31.36          171     GERANYL PROPANOATE                                   0.000   0.753     31.46          172     MW 212 METHYL    0.000   0.000                  ?-DODECENOATE     32.28          177     trans-GERANIOL   0.363   0.180     32.54          179     CAS# 000483-77-2 0.069   0.381                  (CALAMENENE ?)     34.38          187     METHYL 3,6-      0.061   0.000                  DODECDIENOATE ME 79     36.42          196     MW 210 ME 43,54,82                                   0.048   0.135     39.11          211     CARYOPHYLLENE OXIDE                                   0.061   0.235     40.23          221     HUMULENE         0.059   0.109                  MONO-EPOXIDE I     40.28          223     MW222 ME 43,82   0.078     41.07          225     HUMULENE         0.161   0.358                  MONO-EPOXIDE II     41.55          228     HUMULENE         0.073   0.167                  MONO-EPOXIDE III     41.88          231     MW 222 ME 43,79,80                                   0.365   0.847     43.88          242     MW 220 ME 43,79,91                                   0.000   0.455     44.73          246     T-CADINOL        0.101   0.232     46.08          255     T-MUUROLOL       0.062   0.111                  ME 95,121,204     47.50          262     MW 220 ME 41,67,109                                   0.000   0.112                  DIEPOXIDE?     48.69          268     FARNESOL         0.148     ______________________________________

                  APPENDIX 2     ______________________________________     Detailed GLC analyses of Columbus and other U.S. high alpha     ______________________________________     hops.                                        Area %                            COLUMBUS    GALENA     Identification                   Group    92-12-8-2   92-12-8-3     ______________________________________     Apha-Pinene            0.125       0.184     Beta-Pinene   1        0.943       0.883     Myrcene       1        51.571      66.560     Methyl butyl Isobutyrate                            0.838       3.575     Limonene      4        0.288       0.350     Linalool      3        0.470       0.294     Caryophyllene 1        6.297       3.097     Farnesene?    1        0.275       0.112     Humulene      1        11.049      5.764     Muurolene     4        0.768       0.309     Selinene      4        1.472       0.341     Geranyl Acetate                   3        0.016       0.048     delta Cadinene                   4        1.834       0.292     Gamma Cadinene                   4        1.095       0.143     Geranyl Isobutyrate                   3        1.716       0.296     Undecanone-2           1.348       0.059     Geraniol      3        0.048       0.060     Limonen-10-ol          0.155       0.021     Caryophyllene oxide                   2        0.259       0.014     Humulene epoxide I                   2        0.430       0.033     Humulene epoxide II                   2        1.450       0.068     Caryolan-i-ol 2        0.120       0.006     Humulene epoxide III                   2        0.025       0.013     Humulol       2        0.185       0.011     beta Eudesmol?     alpha Eudesmol?     Humulenol II  2        0.103     Humulene diepoxide?                   2     Major Hydrocarbons (1) 70.135      76.216     Oxidation Products (2) 2.572       0.145     Floral-Estery (3)      2.250       0.698     Citrus Piney (4)       5.457       1.435     Total Accounted For    80.414      78.494     Humulene/Caryophyllene 1.753       1.861     ______________________________________                            CHINOOK     NUGGET     Identification                   Group    92-12-9-1   92-12-9-2     ______________________________________     Apha-Pinene            0.047       0.081     Beta-Pinene   1        0.435       0.667     Myrcene       1        28.469      51.972     Methyl butyl Isobutyrate                            1.410       1.274     Limonene      4        0.136       0.183     Linalool      3        0.369       1.022     Caryophyllene 1        9.516       8.597     Farnesene?    1        0.353       0.162     Humulene      1        21.157      19.029     Muurolene     4        1.517       0.921     Selinene      4        1.385       0.818     Geranyl Acetate                   3        0.210       0.044     delta Cadinene                   4        4.215       0.926     Gamma Cadinene                   4        2.686       0.445     Geranyl Isobutyrate                   3        3.323       0.076     Undecanone-2           0.675       0.453     Geraniol      3        0.155       0.079     Limonen-10-ol          0.049       0.037     Caryophyllene oxide                   2        0.076       0.032     Humulene epoxide I                   2        0.326       0.220     Humulene epoxide II                   2        0.663       0.328     Caryolan-i-ol 2        0.088       0.015     Humulene epoxide III                   2        0.033     Humulol       2        0.379       0.040     beta Eudesmol?         0.248     alpha Eudesmol?        0.205     Humulenol II  2        0.112       0.022     Humulene diepoxide?                   2        0.316     Major Hydrocarbons (1) 59.924      80.427     Oxidation Products (2) 1.993       0.657     Floral-Estery (3)      4.057       1.221     Citrus Piney (4)       9.939       3.293     Total Accounted For    75.913      85.598     Humulene/Caryophyllene 2.223       2.213     ______________________________________     Area Percent Report     Sample Name: 92-12-8-2     Pk #  Ret Time Area        Area %                                      Identification     ______________________________________     1     7.030    3884        0.031     2     7.854    1456        0.011     3     8.010    1580        0.012     4     9.053    8364        0.066     5     9.260    10821       0.086     6     9.358    15718       0.125 Alpha-Pinene     7     9.800    4213        0.033     8     10.297   2957        0.023     9     10.543   9679        0.077     10    11.097   967         0.007     11    11.242   118386      0.943 Beta-Pinene     12    11.904   1183        0.009     13    12.476   6468547     51.571                                      Myrcene     14    12.685   4762        0.038     15    12.746   3884        0.031     16    13.064   52106       0.415     17    13.204   48568       0.387     18    13.274   105192      0.838 Methyl butyl Isobutyrate     19    13.586   36238       0.288 Limonene     20    13.797   3396        0.027     21    13.938   26704       0.212     22    14.149   4588        0.036     23    14.333   8748        0.069     24    14.520   1416        0.011     25    14.602   16543       0.131     26    14.879   17328       0.138     27    15.087   7345        0.058     28    15.212   931         0.007     29    15.420   743         0.005     30    15.506   3693        0.029     31    15.722   3398        0.027     32    15.801   10636       0.084     33    16.009   5267        0.042     34    16.069   2526        0.020     35    16.244   25684       0.204     36    16.624   3088        0.024     37    16.793   10279       0.081     38    16.915   3626        0.028     39    17.427   1969        0.015     40    17.673   67587       0.538     41    18.248   2557        0.020     42    18.354   1000        0.008     43    18.423   691         0.005     44    18.953   7042        0.056     45    19.178   7410        0.059     46    19.291   6033        0.048     47    19.466   10779       0.085     48    19.777   7142        0.056     49    19.956   4956        0.039     50    20.134   23743       0.189     51    20.364   804         0.006     52    20.742   2092        0.016     53    20.877   5423        0.043     54    20.984   1676        0.013     55    21.043   923         0.007     56    21.229   29717       0.236     57    21.448   1539        0.012     58    21.532   7494        0.059     59    21.843   8179        0.065     60    22.211   1838        0.014     61    22.295   6856        0.054     62    22.441   19924       0.158     63    22.568   2933        0.023     64    22.750   66342       0.528     65    22.922   5479        0.043     66    23.076   2236        0.017     67    23.224   1919        0.015     68    23.360   6240        0.049     69    23.682   59058       0.470 Linalool     70    23.938   25935       0.206     71    24.171   19672       0.156     72    24.285   1535        0.012     73    24.432   6320        0.050     74    24.542   1797        0.014     75    24.816   10688       0.085     76    25.028   9189        0.073     77    25.276   29524       0.235     78    25.457   3016        0.024     79    25.597   16721       0.133     80    25.737   4624        0.036     81    25.858   45802       0.365     82    26.177   789857      6.297 Caryophyllene     83    26.267   147160      1.173     84    26.483   11876       0.094     85    26.654   2115        0.016     86    26.951   19239       0.153     87    27.128   7807        0.062     88    27.262   34560       0.275 Farnesene?     89    27.452   5485        0.043     90    27.650   12130       0.096     91    27.741   32092       0.255     92    28.029   22059       0.175     93    28.286   1385879     11.049                                      Humulene     94    28.518   174316      1.389     95    28.816   1217        0.009     96    29.099   46594       0.371     97    29.289   65255       0.520     98    29.452   50235       0.400     99    29.663   101538      0.809     100   29.743   96334       0.768 Muurolene     101   29.922   184741      1.472 Selinene     102   30.066   2003        0.016 Geranyl Acetate     103   30.168   16997       0.135     104   30.380   230089      1.834 delta Cadinene     105   30.604   137367      1.095 Gamma Cadiene     106   30.702   18692       0.149     107   30.952   9850        0.078     108   31.085   4796        0.038     109   31.312   215300      1.716 Geranyl Isobutyrate     110   31.411   169139      1.348 Undecanone-2     111   31.517   26715       0.213     112   31.630   77466       0.617     113   31.737   5913        0.047     114   31.821   6115        0.048 Geraniol     115   32.106   9621        0.076     116   32.224   20686       0.164     117   32.501   13220       0.105     118   32.620   30790       0.245     119   33.031   7712        0.061     120   33.254   2445        0.019     121   33.462   7790        0.062     122   33.722   28110       0.224     123   33.960   4270        0.034     124   34.081   2094        0.016     125   34.192   4256        0.033     126   34.312   4289        0.034     127   34.372   5764        0.046     128   34.483   5031        0.040     129   34.565   5092        0.040     131   34.901   34301       0.273     132   35.009   13488       0.107     133   35.126   5975        0.047     134   35.299   1614        0.012     135   35.487   11667       0.093     136   35.854   17035       0.135 Limonen-10-ol     137   36.161   2233        0.017     138   36.312   32546       0.259 Caryophyllene oxide     139   36.476   1067        0.008     140   36.577   2462        0.019     141   36.935   6949        0.055     142   37.071   1073        0.008     143   37.206   13125       0.104     144   37.370   2081        0.016     145   37.555   7101        0.056     146   37.672   54047       0.430 Humulene epoxide I     147   38.006   8150        0.065     148   38.127   3162        0.025     149   38.444   2423        0.019     150   38.688   30971       0.246     151   38.915   181869      1.450 Humulene epoxide II     152   39.075   15063       0.120 Caryolan-1-ol     153   39.222   3225        0.025 Humulene epoxide III     154   39.501   7444        0.059     155   39.809   1283        0.010     156   39.935   1171        0.009     157   40.158   5912        0.047     158   40.396   1243        0.009     159   40.677   52277       0.416     160   40.871   8343        0.066     161   41.254   6706        0.053     162   41.404   23253       0.185 Humulol     163   41.759   4806        0.038     164   41.946   30832       0.245     165   42.077   2776        0.022     166   42.236   2521        0.020     167   42.537   2687        0.021     168   42.964   11140       0.088     169   43.065   7509        0.059     170   43.258   23800       0.189     171   43.848   4438        0.035     172   44.637   12985       0.103 Humulenol II     173   45.039   5691        0.045     174   45.892   3866        0.030     175   46.597   1815        0.014     176   47.014   2417        0.019     177   50.412   1109        0.008     = 1.25428E + 007     ______________________________________     Area Percent Report     Sample Name: 92-12-8-3     Pk #  Ret Time Area        Area %                                      Identification     ______________________________________     1     7.034    2371        0.022     2     7.855    753         0.007     3     7.988    864         0.008     4     8.623    706         0.066     5     9.056    1684        0.016     6     9.262    2429        0.023     7     9.359    19367       0.184 alpha Pinene     8     9.801    6546        0.062     9     10.096   924         0.008     10    10.318   10977       0.104     11    10.547   49458       0.469     12    10.920   660         0.006     13    11.098   1561        0.014     14    11.242   93039       0.883 beta Pinene     15    11.733   1061        0.010     16    11.901   3162        0.030     17    12.090   1322        0.012     18    12.484   6985773     66.360                                      Myrcene     19    12.692   7230        0.068     20    12.750   5559        0.052     21    13.073   155982      1.481     22    13.217   83592       0.794     23    13.301   376416      3.575 Methylbutyl Isobutyrate     24    13.593   36853       0.350 Limoene     25    13.810   1808        0.017     26    13.942   30257       0.287     27    14.151   2083        0.019     28    14.317   15305       0.145     29    14.529   2092        0.019     30    14.609   73712       0.700     31    14.896   239176      2.272     32    15.091   5970        0.056     33    15.333   699         0.006     34    15.508   2556        0.024     35    15.713   3795        0.036     36    15.804   17078       0.162     37    16.011   59153       0.561     38    16.246   26755       0.254     39    16.818   12944       0.123     40    17.427   1728        0.016     41    17.682   151069      1.435     42    18.253   1689        0.016     43    18.730   932         0.008     44    18.953   1326        0.012     45    19.192   122003      1.159     46    19.329   5927        0.056     47    19.481   11385       0.108     48    19.791   7946        0.075     49    19.952   1333        0.012     50    20.134   5804        0.055     51    20.727   9706        0.092     52    20.879   21447       0.203     53    21.054   2750        0.026     54    21.227   17557       0.166     55    21.769   2599        0.024     56    22.298   42843       0.407     57    22.437   4686        0.044     58    22.494   4842        0.046     59    22.739   15483       0.147     60    22.234   720         0.006     61    23.358   6212        0.059     62    23.681   30996       0.294 Linalool     63    23.937   41114       0.390     64    24.168   9021        0.085     65    24.789   2293        0.021     66    25.025   2599        0.024     67    25.343   82950       0.788     68    25.449   1561        0.014     69    25.596   28078       0.266     70    25.830   3277        0.031     71    26.073   326058      3.097 Caryophyllene     72    26.267   160273      1.522     73    26.482   8286        0.078     74    26.695   887         0.008     75    26.833   1478        0.014     76    26.923   20836       0.197     77    27.019   1307        0.012     78    27.112   1737        0.016     79    27.223   11802       0.112 Farnesene?     80    27.440   2763        0.026     81    27.709   6688        0.063     82    28.023   24366       0.231     83    28.227   606830      5.764 Humulene     84    28.353   4325        0.041     85    28.485   24982       0.237     86    28.582   4950        0.047     87    28.782   837         0.008     88    29.082   8038        0.076     89    29.251   9309        0.088     90    29.429   7845        0.074     91    29.642   32547       0.309 Muurolene     92    29.716   35968       0.341 Selinene     93    29.883   5145        0.048 Geranyl Acetate     94    30.139   3103        0.029     95    30.328   30746       0.292 delta Cadinene     96    30.562   15061       0.143 gamma Cadinene     97    30.780   1170        0.011     98    31.068   2479        0.023     99    31.144   7554        0.071     100   31.383   31189       0.296 Geranyl Isobutyrate     101   31.504   6271        0.059 Undecanone-2     102   31.610   3590        0.034     103   31.720   6384        0.060 Geraniol     104   32.097   2303        0.021     105   32.222   1438        0.013     106   32.489   10866       0.103     107   32.608   3493        0.033     108   33.028   780         0.007     109   33.719   42378       0.402     110   33.949   697         0.006     111   34.181   786         0.007     112   34.478   1455        0.013     113   34.754   699         0.006     114   34.891   1927        0.018     115   35.004   1561        0.014     116   35.121   616         0.005     117   35.487   19190       0.182     118   35.892   2261        0.021 Limonen-10-ol     119   36.302   1551        0.014 Caryophyllene oxide     120   37.203   2536        0.024     121   37.660   3554        0.033 Humulene epoxide I     122   38.680   5605        0.053     123   38.888   7257        0.068 Humulene epoxide II     124   39.082   690         0.006 Caryolan-1-ol     125   40.154   1386        0.013 Humulene epoxide III     126   40.669   3261        0.031     127   40.872   1801        0.017     128   41.248   3412        0.032     129   41.399   1245        0.011 Humulol     130   41.937   4179        0.039     131   43.258   1708        0.016     Total area = 1.0527E + 007     ______________________________________     Area Percent Report     Sample Name: 92-12-9-1     Pk #  Ret Time Area        Area %                                      Identification     ______________________________________     1     7.028    3603        0.029     2     7.848    1142        0.009     3     8.004    772         0.006     4     9.048    3120        0.025     5     9.253    7251        0.060     6     9.351    5689        0.047 alpha Pinene     7     9.794    4017        0.033     8     10.302   1522        0.012     9     10.538   14530       0.120     10    11.090   761         0.006     11    11.231   52365       0.435 beta Pinene     12    11.886   700         0.005     13    12.412   3424969     28.463                                      Myrcene     14    12.648   2525        0.021     15    12.725   2971        0.024     16    13.048   23622       0.196     17    13.193   43283       0.359     18    13.268   169688      1.410 Methylbutyl Isobutyrate     19    13.576   16461       0.136 Limonene     20    13.794   2031        0.016     21    13.929   18137       0.150     22    14.311   4573        0.038     23    14.595   10186       0.084     24    14.873   11403       0.094     25    15.080   12395       0.103     26    15.413   700         0.005     27    15.501   899         0.007     28    15.700   2332        0.019     29    15.796   15536       0.129     30    16.002   9137        0.075     31    16.243   38778       0.322     32    16.787   5760        0.047     33    16.906   1286        0.010     34    17.425   608         0.005     35    17.676   114830      0.954     36    18.247   625         0.005     37    18.947   1621        0.013     38    19.175   28172       0.234     39    19.294   3311        0.027     40    19.463   9984        0.083     41    19.771   2700        0.022     42    19.947   1731        0.014     43    20.130   4426        0.036     44    20.732   2604        0.021     45    20.874   10809       0.089     46    21.044   2047        0.017     47    21.230   61271       0.509     48    21.533   14424       0.119     49    21.780   1521        0.012     50    21.843   956         0.007     51    22.211   2063        0.017     52    22.293   15179       0.126     53    22.443   32264       0.268     54    22.570   3366        0.028     55    22.756   106737      0.887     56    23.080   5302        0.044     57    23.356   6309        0.052     58    23.680   44440       0.369 Linalool     59    23.941   75567       0.628     60    24.170   15675       0.130     61    24.434   2150        0.017     62    24.524   1616        0.013     63    24.806   7293        0.060     64    25.028   2541        0.021     65    25.278   37278       0.309     66    25.346   31734       0.263     67    25.605   32557       0.270     68    25.881   90397       0.751     69    26.140   1145130     9.516 Caryophyllene     70    26.269   176130      1.463     71    26.486   9543        0.079     72    26.835   1812        0.015     73    26.988   22481       0.186     74    27.140   17473       0.145     75    27.353   42477       0.353 Farnesene?     76    27.473   5304        0.044     77    27.783   61915       0.514     78    28.035   24287       0.201     79    28.343   2545867     21.157                                      Humulene     80    28.547   315497      2.622     81    28.832   2334        0.019     82    28.945   5985        0.049     83    29.120   75042       0.623     84    29.300   116317      0.966     85    29.469   97160       0.807     86    29.682   182550      1.517 Muurolene     87    29.764   166698      1.385 Selinene     88    29.967   25357       0.210 Geranyl Acetate     89    30.169   7062        0.058     90    30.417   507237      4.215 delta Cadiene     91    30.630   323297      2.686 gamma Cadiene     92    30.879   5404        0.044     93    31.069   10371       0.086     94    31.155   5319        0.044     95    31.332   399851      3.323 Geranyl Isoburyrate     96    31.467   81272       0.675 Undecanone-2     97    31.617   7782        0.064     98    31.730   18703       0.155 Geraniol     99    31.890   7540        0.062     100   32.109   8984        0.074     101   32.223   86628       0.719     102   32.436   9265        0.077     103   32.491   6647        0.055     104   32.620   30270       0.251     105   33.035   3306        0.027     106   33.230   743         0.006     107   33.464   4214        0.035     108   33.728   87090       0.723     109   33.951   2444        0.020     110   34.183   3253        0.027     111   34.370   4703        0.039     112   34.480   4897        0.040     113   34.758   3342        0.027     114   34.896   11595       0.096     115   35.007   14221       0.118     116   35.299   1278        0.010     117   35.489   39880       0.331     118   35.873   5944        0.049 Limonen-10-ol     119   35.982   1196        0.009     120   36.163   3016        0.025     121   36.310   9199        0.076 Caryophyllene oxide     122   36.564   1474        0.012     123   36.871   5580        0.046     124   37.205   13593       0.113     125   37.365   21562       0.179     126   37.551   3095        0.025     127   37.669   39322       0.326 Humulene epoxide I     128   37.863   1467        0.012     129   38.004   7647        0.063     130   38.124   2074        0.017     131   38.438   1793        0.014     132   38.685   50800       0.422     133   38.900   79889       0.663 Humulene epoxide II     134   39.082   10671       0.088 Caryolan-1-ol     135   39.209   4042        0.033 Humulene epoxide III     136   39.500   2852        0.023     137   39.716   694         0.005     138   39.789   600         0.005     139   40.149   3072        0.025     140   40.465   1310        0.010     141   40.672   16151       0.134     142   40.874   13029       0.108     143   41.250   10136       0.084     144   41.406   45643       0.379 Humulol     145   41.758   11528       0.095     146   41.846   13183       0.109     147   41.945   25814       0.214     148   42.073   9818        0.081     149   42.534   5222        0.043     150   42.964   29878       0.248 beta Eudesmol?     151   43.065   24765       0.205 alpha Eudesmol?     152   43.255   33781       0.280     153   43.565   1828        0.015     154   43.846   10003       0.083     155   44.289   5485        0.045     156   44.370   3097        0.025     157   44.633   13527       0.112 Humulenol II     158   45.042   12525       0.104     159   45.700   38097       0.316 Humulene diepoxide?     160   45.885   3830        0.031     161   46.615   2140        0.017     162   47.030   2967        0.024     163   48.254   4136        0.034     164   48.524   1729        0.014     165   52.198   1275        0.010     Total area = 1.20327E + 007     ______________________________________     Area Percent Report     Sample Name: 92-12-9-2     Pk #  Ret Time Area        Area %                                      Identification     ______________________________________     1     7.034    1627        0.013     2     7.850    1222        0.010     3     9.051    1362        0.011     4     9.259    2214        0.018     5     9.355    9693        0.081 alpha Pinene     6     9.796    3741        0.031     7     10.309   1970        0.016     8     10.542   19685       0.166     9     11.093   943         0.008     10    11.236   79174       0.667 beta Pinene     11    11.895   2296        0.019     12    12.468   6160576     51.972                                      Myrcene     13    12.679   3749        0.031     14    12.741   4270        0.036     15    13.058   44822       0.378     16    13.201   59258       0.499     17    13.275   151104      1.274 Methylbutyl Isobutyrate     18    13.582   21713       0.183 Limonene     19    13.800   1814        0.015     20    13.934   28562       0.241     21    14.144   933         0.007     22    14.311   6730        0.056     23    14.519   2088        0.017     24    14.609   6243        0.052     25    14.875   55333       0.466     26    15.083   3970        0.033     27    15.326   1045        0.008     28    15.416   636         0.005     29    15.503   2028        0.017     30    15.702   2642        0.022     31    15.798   10210       0.086     32    16.005   54321       0.458     33    16.241   21435       0.180     34    16.809   7970        0.067     35    17.438   1939        0.016     36    17.655   61020       0.514     37    17.783   1252        0.010     38    18.248   665         0.005     39    18.948   1086        0.009     40    19.179   60530       0.510     41    19.314   1798        0.015     42    19.462   8464        0.071     43    19.546   4332        0.036     44    19.785   6451        0.054     45    19.949   1390        0.011     46    20.130   4840        0.040     47    20.722   11806       0.099     48    20.873   1283        0.010     49    20.945   2757        0.023     50    21.226   4405        0.037     51    21.524   2418        0.020     52    21.776   2520        0.021     53    22.199   1123        0.009     54    22.294   37518       0.316     55    22.438   12669       0.106     56    22.558   1478        0.012     57    22.743   43371       0.365     58    23.076   1222        0.010     59    23.236   1059        0.008     60    23.355   8797        0.074     61    23.681   121220      1.022 Linalool     62    23.935   17913       0.151     63    24.167   9930        0.083     64    24.417   1477        0.012     65    24.798   946         0.008     66    24.923   911         0.007     67    25.023   2303        0.019     68    25.347   50189       0.423     69    25.462   730         0.006     70    25.605   63484       0.535     71    25.731   14566       0.122     72    25.863   19067       0.160     73    26.129   1019106     8.597 Caryophyllene     74    26.267   172193      1.452     75    26.484   13509       0.114     76    26.649   3488        0.029     77    26.830   1518        0.012     78    26.973   2085        0.017     79    37.057   3159        0.026     80    27.127   4597        0.038     81    27.284   19285       0.162 Farnesene?     82    27.452   4597        0.038     83    27.558   1014        0.008     84    27.766   17735       0.149     85    28.030   20185       0.170     86    28.324   2255638     19.029                                      Humulene     87    28.509   62699       0.529     88    28.575   78837       0.665     89    28.933   16176       0.136     90    29.101   26411       0.222     91    29.319   43633       0.368     92    29.442   23012       0.194     93    29.658   109275      0.921 Muurolene     94    29.741   97039       0.818 Selinene     95    29.881   5248        0.044 Geranyl Acetate     96    30.142   4602        0.038     97    30.351   109776      0.926 delta Cadinene     98    30.580   52832       0.445 gamma Cadinene     99    30.771   1506        0.012     100   30.851   1737        0.014     101   31.082   5775        0.048     102   31.205   11532       0.097     103   31.330   9041        0.076 Geranyl isobutyrate     104   31.511   53799       0.453 Undecanone-2     105   31.717   9415        0.079 Geraniol     106   31.814   3048        0.025     107   32.085   5681        0.047     108   32.219   5853        0.049     109   32.492   1416        0.011     110   32.608   7265        0.061     111   33.033   906         0.007     112   33.253   722         0.006     113   33.712   10903       0.092     114   33.945   1308        0.011     115   34.071   2438        0.020     116   34.181   1069        0.009     117   34.368   5360        0.045     118   34.474   674         0.005     119   34.889   9616        0.081     120   35.001   4520        0.038     121   35.481   8543        0.072     122   35.853   4382        0.037 Limonen 10-ol     123   35.976   1266        0.010     124   36.154   1163        0.009     125   36.296   3788        0.032 Caryophyllene oxide     126   36.909   1692        0.014     127   37.183   9203        0.077     128   37.358   1754        0.014     129   37.660   26083       0.220 Humulene epoxide I     130   37.996   2405        0.020     131   38.673   14395       0.121     132   38.889   38989       0.328 Humulene epoxide II     133   39.079   1867        0.015 Caryolan-1 ol     134   39.922   885         0.007     135   40.138   1296        0.010     136   40.666   11481       0.096     137   40.870   4099        0.034     138   41.245   7744        0.065     139   41.397   4776        0.040 Humulol     140   41.934   9925        0.083     141   43.059   2394        0.020     142   43.254   5249        0.044     143   43.842   1927        0.016     144   44.637   2619        0.022 Humulenol II     = 1.18535E + 007     ______________________________________     Reference:            Code     Variety            92-12-8-2                     Columbus            92-12-8-3                     Galena            92-12-9-1                     Chinook            92-12-9-2                     Nugget      Analytical Conditions: See, Nickerson and van Engel, "Hop Aroma Component      Profile and the Aroma Unit," Journal American Society of Brewing Chemists      50(3), 7782(1992). 

What is claimed is:
 1. A new and distinct variety of hop plant named Columbus, substantially as shown and described. 